Anti-RAD54L2 antibody

供应商 ：江西江蓝纯生物试剂有限公司

库存 ：大量

克隆性 ：多克隆

保质期 ：1年

抗体英文名 ：Anti-RAD54L2 antibody

宿主 ：Rabbit

浓度 ：0.5 mg/ml

保存条件 ：-20°C

规格 ：25 μl/100 μl/200 μl

Anti-RAD54L2 antibody

Cat. No.     JLC221190        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-RAD54L2 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Fusion protein of human RAD54L2
Reactivity                 Human, Mouse
Content                     0.5 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 RAD54L2
Full name                 RAD54-like 2 (S. cerevisiae)
Synonyms    ARIP4; HSPC325; SRISNF2L
Swissprot    Q9Y4B4

Target Background
Adrenergic receptors (ARs) include four general types (a1, a2, b1 and b2) that are found in different target tissues and differ in their affinities and responses to various agonists and antagonists. The coupling of ARs to specific intracellular effectors is mediated through diverse heterotrimeric G proteins. ARs play a critical role in the development of prostate cancer, and transcriptional activity of AR is partly regulated by coregulatory proteins. RAD54L2 (RAD54-like 2), also known as ARIP4 (androgen receptor-interacting protein 4), HSPC325 or SRISNF2L, is a 1,467 amino acid nuclear protein belonging to the SNF2/RAD54 helicase family that consists of one helicase ATP-binding domain and a helicase C-terminal domain. RAD54L2 is a DNA helicase that regulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. RAD54L2 is post-translationally sumoylated or phosphorylated upon DNA damage.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm
Positive control: Human thyroid cancer
Recommended dilution: 50-200    
            
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ml221190(RAD54L2 Antibody) at dilution 1/60, on the right is treated with fusion protein. (Original magnification: ×200)    
Western blotting
Predicted band size:163 kDa
Positive control:Hela, Raji , 293T and PC3 
Recommended dilution: 200-1000

Gel: 6%SDS-PAGE
Lysate: 40 μg
Lane 1-4: Hela cells, Raji cells, 293T cells, PC3 cells
Primary antibody: ml221190(RAD54L2 Antibody) at dilution 1/400
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 5 minutes
    
ELISA
Recommended dilution: 2000-5000