Anti-MCM7 antibody

供应商 ：江西江蓝纯生物试剂有限公司

库存 ：大量

克隆性 ：多克隆

保质期 ：1年

抗体英文名 ：Anti-MCM7 antibody

宿主 ：Rabbit

浓度 ：0.2 mg/ml

保存条件 ：-20°C

规格 ：25 μl/100 μl/200 μl

Anti-MCM7 antibody

Cat. No.     JLC220965        
Package    25 μl/100 μl/200 μl
Storage    -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Product overview
Description                   Anti-MCM7 rabbit polyclonal antibody
Applications        ELISA, WB, IHC 
Immunogen               Fusion protein of human MCM7
Reactivity                 Human, Mouse
Content                     0.2 mg/ml
Host species               Rabbit
Ig class                  Immunogen-specific rabbit IgG
Purification                 Antigen affinity purification

Target information
Symbol                 MCM7
Full name                 minichromosome maintenance complex component 7
Synonyms    MCM2, CDC47, P85MCM, P1CDC47, PNAS146, P1.1-MCM3
Swissprot    P33993

Target Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Applications
Immunohistochemistry
Predicted cell location: Cytoplasm, Nucleus
Positive control: Human ovarian cancer
Recommended dilution: 15-50    Predicted cell location: Cytoplasm, Nucleus
Positive control: Human colon cancer
Recommended dilution: 15-50
            
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using ml220965(MCM7 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200)    The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using ml220965(MCM7 Antibody) at dilution 1/15, on the right is treated with fusion protein. (Original magnification: ×200)
Western blotting
Predicted band size:81 kDa
Positive control:Jurkat cells 
Recommended dilution: 500-2000

Gel: 10%SDS-PAGE
Lysate: 50 μg
Lane: Jurkat cells
Primary antibody: ml220965(MCM7 Antibody) at dilution 1/300
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution
Exposure time: 30 seconds
    
ELISA
Recommended dilution: 1000-5000