Mouse FAD104 / FNDC3B ELISA Kit (Sandwich ELISA) - LS-F33919

价格： $1090

品牌：LSBio

货号：LS-F33919

产品详情

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Product Description

LS-F33919 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse FAD104 / FNDC3B in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of FAD104 / FNDC3B as low as 0.188 nanograms per millilter.
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Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

FAD104 / FNDC3B
Synonyms

FNDC3B, FAD104, HCV NS5A-binding protein 37, NS5ABP37, YVTM2421, PRO4979
Reactivity

Mouse
Manual

Intended Sample Types

Plasma, Serum, Tissue Homogenates
Format

96-Well Strip Plate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

0.312 - 20 ng/ml
Sensitivity

0.188 ng/ml
Precision

Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage

Store at 4°C. Stable for 6 months.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

User Manual
96-well Microplate
Detection Reagents
Wash Buffer
Substrate
Stop Solution
Adhesive Plate Sealers

Background

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Fndc3b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Fndc3b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Fndc3b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofFndc3b can be calculated.
Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
About FAD104 / FNDC3B

May be a positive regulator of adipogenesis. Uniprot: Q53EP0 NCBI: NM_022763 NP_073600.3

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