RNA酶抑制剂

库存 ：大量

英文名 ：RNA酶抑制剂

CAS号 ：wlb1378

保质期 ： 2年

供应商 ： 瓦兰生物

保存条件 ：常温

规格 ：1000U/2000U/10000U

RNA酶抑制剂
产品简介

规格	1000U ; 2000U ; 10000U
外观（性状）	液体
储存条件	-20℃
单位	支

Description RNasin Ribonuclease Inhibitors have broad-spectrum RNase inhibitory properties, including inhibition of eukaryotic RNases of the neutral type. The 50kDa protein exerts its inhibitory effect by noncovalently binding to RNases at a 1:1 ratio. The Ki value for the binding of RNasin Ribonuclease Inhibitor to RNase (e.g., RNase A) is approximately 10-14M. In addition, the kinetics of association for RNasin Ribonuclease Inhibitor is very rapid, ensuring immediate complexing and inhibition of RNase. RNasin Ribonuclease Inhibitors is purified using a combination of ion exchange and affinity chromatography. They are devoid of DNA exonuclease and endonuclease activity and RNase activity. Isolated from a recombinant E. coli strain, the N-terminus is an unblocked serine residue. Unit Definition: One unit is defined as the amount of RNasin Ribonuclease Inhibitor required to inhibit the activity of 5ng of ribonuclease A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2´,3´-cyclic monophosphate by ribonuclease A. Enzyme Storage Buffer: RNasin Ribonuclease Inhibitor is supplied in 20mM HEPES-KOH (pH 7.6), 50mM KCl, 8mM DTT, 50% (v/v) glycerol. General Considerations Since ribonucleases typically retain activity under denaturing conditions, care must be taken to avoid denaturing RNasin Ribonuclease Inhibitor molecules that have complexed with ribonuclease. To prevent the release of active ribonuclease, temperatures greater than 50°C and high concentrations of urea or other denaturing agents should be avoided. RNasin Ribonuclease Inhibitors are active over a broad pH range. If diluted and stored for extended periods of time, include DTT (minimum concentration 1mM). Usage Notes: RNasin Ribonuclease Inhibitor is active over a broad pH range. Concentration gradients may form in frozen products and should be dispersed upon thawing. Mix well prior to use. Contaminant A ctivity 1. RNase Assays: No RNA degradation is detected. 2. DNase Assay: No DNA degradation is detected. 3. Endonuclease Assay: No DNA degradation is detected. 4. Physical Purity: The purity is >90% as judged by SDS-polyacrylamide gels. Effectiveness of RNasin Ribonuclease Inhibitor A gainst Selected Nucleases. Inhibits Does Not Inhibit RNase A RNase T1 RNase B S1 Nuclease RNase C RNase from As pergil lus sp. human placental RNase RNase H, RNase ONE Ribonuclease, Taq DNA polymerase, ImProm-II,AMV or M-MLV Reverse Transcriptase, SP6, T7 or T3 RNA polymerase