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样本 :血清/血浆/组织匀浆液
库存 :1000
适应物种 :Chicken
应用 :科研使用
检测方法 :ELISA
检测限 :40 - 1400 pg/ml
供应商 :康朗生物
规格 :96T
Chicken Estradiol ELISA Kit (Competitive EIA) - LS-F10110Catalog
Size
Price
LS-F10110-1
1 plate
$715 USD
Available Discounts
10% off 5+ units
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Product Description
LS-F10110 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Chicken Estradiol in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Competitive EIA assay principle and can be used to detect levels of Estradiol as low as 25 picograms per milliliter.More Estradiol Products
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Specifications
TypeCompetitive EIA (enzyme immunoassay) kit
Target
Estradiol
Reactivity
Chicken
Specificity
This assay has high sensitivity and excellent specificity for detection of chicken E2. No significant cross-reactivity or interference between chicken E2 and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between chicken E2 and all the analogs, therefore, cross reaction may still exist.
Manual
Intended Sample Types
Plasma, Serum, Tissue Homogenates
Sample Size
50 - 100 µl
Format
96-Well Microplate
Detection
Colorimetric - 450nm (TMB)
Measurement
Quantitative
Detection Range
40 - 1400 pg/ml
Sensitivity
25 pg/ml
Precision
Intra-assay: CV%<15% Inter-assay: CV%<15%
Storage
Short term: 4°C; Long term: see manual.
Quality Assurance
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components
- User Manual
- 96-well Microplate
- Detection Reagents
- Wash Buffer
- Substrate
- Stop Solution
- Adhesive Plate Sealers
Background
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Restrictions
For research use only.
Guarantee
This elisa kit carries the LSBio 100% Guarantee.
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入驻年限:10年