具有低凝胶温度的琼脂糖

价格： ￥571.72

品牌：SIGMA

货号：A9414

产品详情

性质

Related Categories	Agarose, Agarose Gel, Core Bioreagents, Nucleic Acid Electrophoresis, Research Essentials, 更多...
grade	for molecular biology
product line	BioReagent
EEO	≤0.10
mp	≤65 °C ( at a 1.5% gel)
transition temp	congealing temperature 26-30 °C
gel strength	≥200 g/cm² (1% gel)
anion traces	sulfate (SO₄^2-): ≤0.10%
foreign activity	DNase, RNase, NICKase, none detected

产品描述

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Application

本品经过改性，凝胶温度和熔点更低，便于从低熔点琼脂糖凝胶中回收和纯化 DNA。使用从低熔点琼脂糖凝胶上切下的 DNA 凝胶薄片可直接在重熔琼脂糖中制备放射性标记的 DNA 探针。
使用从低熔点琼脂糖凝胶上切下的 DNA 凝胶薄片可直接在重熔琼脂糖中方便地将限制性片段克隆到质粒中。
使用了溴化乙锭染料，因此凝胶具有低背景荧光。