Mouse EGF ELISA Kit (Sandwich ELISA) - LS-F235

价格： $590

品牌：LSBio

货号：LS-F235

产品详情

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Product Description

LS-F235 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Mouse EGF in samples of Cell Culture Supernatants, Plasma and Serum. It is based upon a Sandwich assay principle and can be used to detect levels of EGF as low as 3.277 picograms per milliliter.
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Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

EGF
Synonyms

EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Reactivity

Mouse
Specificity

The antibody pair provided in this kit recognizes mouse EGF.
Manual

Intended Sample Types

Cell Culture Supernatants, Plasma, Serum
Sample Dilution

20-100 fold
Format

96-Well Microplate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

3.277 - 320 pg/ml
Sensitivity

3.277 pg/ml
Precision

Intra-Assay: CV<10% Inter-Assay: CV<12%
Storage

Short term: 4°C; Long term: see manual.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

Coated 96-well Strip Plate
Wash Buffer Concentrate (20X)
Standard (Lyophilized)
Biotinylated Detection Antibody
HRP-Streptavidin Conjugate (200x)
TMB One-Step Substrate Reagent
Stop Solution
Assay Diluent A
Assay Diluent B (5x)
Adhesive Plate Sealers

Protocol

Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
About EGF

EGF is a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Uniprot: P01133 NCBI: NM_001963 NP_001954.2

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