低密度脂蛋白受体相关蛋白9抗体规格

低密度脂蛋白受体相关蛋白9抗体规格

价格: 询价

品牌:上海博湖

货号:BH-K22729

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抗体名 :低密度脂蛋白受体相关蛋白9抗体规格

抗体英文名 :Anti-SorLA/LRP9

靶点 :详见说明书

浓度 :1mg/1ml

应用范围 :ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500

宿主 :详见说明书

供应商 :上海博湖

库存 :大量

级别 :详见说明书

目录编号 :详见说明书

抗原来源 :Rabbit

保质期 :详见说明书

适应物种 :详见说明书

标记物 :详见说明书

克隆性 :多克隆

保存条件 :Store at -20 °C

形态 :详见说明书

亚型 :IgG

免疫原 :KLH conjugated synthetic peptide derived from human SorLA/LRP9

规格 :0.2ml/200μg

英文名称  Anti-SorLA/LRP9
中文名称 低密度脂蛋白受体相关蛋白9抗体规格 
     C11orf32; FLJ21930; FLJ39258; gp250; LDLR relative with 11 ligand binding repeats; LDLR relative with 11 ligand-binding repeats; Low density lipoprotein receptor relative with 11 ligand binding repeats; Low-density lipoprotein receptor relative with 11 ligand-binding repeats; LR 11; LR11; LRP 9; LRP9; Mosaic protein LR11; SORL 1; SORL_HUMAN; SORL1; SorLA 1; SorLA; SorLA-1; Sortilin related receptor; Sortilin related receptor L(DLR class) A repeats containing; Sortilin-related receptor; Sorting protein related receptor containing LDLR class A repeats; Sorting protein-related receptor containing LDLR class A repeats; 


低密度脂蛋白受体相关蛋白9抗体规格      1mg/1ml
 0.2ml/200μg
抗体来源  Rabbit
克隆类型   polyclonal
交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
产品类型    一抗  
研究领域    细胞生物 神经生物学 细胞膜蛋白
蛋白分子量  predicted molecular weight: 235kDa
     Lyophilized or Liquid
  KLH conjugated synthetic peptide derived from human SorLA/LRP9
     IgG
纯化方法   affinity purified by Protein A
  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4


低密度脂蛋白受体相关蛋白9抗体规格产品应用  ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 Sortilin-related receptor, also known as Sorting protein-related receptor containing LDLR class A (SorLA), is a Type I membrane protein that may be involved in cell-cell interaction. SorLA, a single transmembrane receptor, binds LDL (the main cholesterol-carrying lipoprotein of plasma) and transports it into cells by endocytosis. SorLA is synthesized as a proreceptor which is processed to the mature form by a furin-like propeptidase. It can also bind to RAP, receptor-associated protein. SorLa is a multifunctional endocytis receptor important in lipoprotein and protease uptake. The N-terminal propeptide, which is removed, can be cleaved by furin or homologous proteases. Endogenous sorLA binds the neuropeptide head activator (HA) and is important for HA signaling and function. It is expressed mainly in brain (cerebral cortex, cerebellum and the occipital pole), but can also be found in liver, spinal cord, kidney, testis and pancreas.

Function : Likely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction.
Subunit : Belongs to the SORL1 family. Contains 5 BNR repeats. Contains 1 EGF-like domain. Contains 6 fibronectin type-III domains. Contains 11 LDL-receptor class A domains. Contains 5 LDL-receptor class B repeats.
Subcellular Location : Membrane.
Tissue Specificity : Expressed mainly in brain, where it is most abundant in the cerebellum, cerebral cortex and the occipital pole; low expression in the putamen and the thalamus. According to PubMed:9157966, found in spinal cord, testis, liver, kidney and pancreas with detectable levels in placenta, lung and heart. According to PubMed:8940146, expressed in the prostate, ovary, thyroid and spleen, but not found in kidney, liver, lung, skeletal muscle, bone marrow and adrenals.
Post-translational modifications : The propeptide removed in the N-terminus may be cleaved by furin or homologous proteases.
Similarity : Belongs to the VPS10-related sortilin family. SORL1 subfamily.
Contains 5 BNR repeats.
Contains 1 EGF-like domain.
Contains 6 fibronectin type-III domains.
Contains 11 LDL-receptor class A domains.
Contains 5 LDL-receptor class B repeats.


低密度脂蛋白受体相关蛋白9抗体规格抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,ElisaWB,免疫荧光等实验。
低密度脂蛋白受体相关蛋白9抗体规格实验原理 :
1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
2)活补体:IgMIgG1IgG2IgG3可通过经典途径激活补体,凝聚的IgAIgG4IgE可通过替代途径激活补体。
3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
4)可通过胎盘及粘膜:免疫球蛋白GIgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白AIgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,6070即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。


Anti-TNFAIP1/FITC 荧光素标记坏死因子α诱导蛋白1抗体IgGMulti-class antibodies规格: 0.2ml
PDGF-R-A 血小板源性生长因子受体-A(抗原)Multi-class antibodies规格: 0.5mg
金属蛋白酶组织抑制因子-4抗体 Anti-TIMP-4 0.1ml
5HT3D Receptor 英文名称: 5-羟受体3D抗体 0.2ml
ENAH 英文名称: ENAH蛋白抗体 0.2ml
Rhesus antibody Rh phospho-KCND2/Kv4.2(Thr602) 化电压门控性通道蛋白Kv4.2抗体 规格 0.1ml
PDGF-R-A 血小板源性生长因子受体-A(抗原)Multi-class antibodies规格: 0.5mg
phospho-p38 ERK/MAPK14 (pThr180/pTyr182) peptide 化原活化蛋白激酶p38抗原Multi-class antibodies规格: 0.5mg

Anti-MASH1 神经母细胞特异性转移因子抗体Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh phospho-AQP2(Ser269) 化水通道蛋白2抗体 规格 0.1ml
Tgase 浓缩液 0.1ml 进口分装
UEVLD 英文名称: 泛素结合酶E2样蛋白抗体 0.2ml
DIRC2 英文名称: 干扰肾细胞癌蛋白2抗体 0.2ml
Anti-MASH1 神经母细胞特异性转移因子抗体Multi-class antibodies规格: 0.1ml
5HTR2C 英文名称: 5-羟受体2C抗体 0.2ml

Ephrin A5 英文名称: 酪酸蛋白激酶A5受体抗体 0.1ml
细胞因子信号传导抑制蛋白1抗体 Anti-Socs 1 0.1ml
Anti-TFR/CD71/FITC 荧光素标记转铁蛋白受体抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh Phospho-MAPKAPK2(Thr334) 化原活化蛋白激酶活化的蛋白激酶2抗体 规格 0.1ml
neurofasciu-155 神经束蛋白-155Multi-class antibodies规格: 0.5mg
YPD琼脂250g用于测定酵母菌总数(GB标准)

改良Skirrow氏琼脂基础 250(g) incubation media 改良Skirrow氏琼脂基础 250(g)
M-肠球菌琼脂 M-Enerococcus Agar 250 用于滤膜法或直接平板法,从自来水、食品或其他标本中分离肠球菌
録三糖铁250用于副溶血性弧菌的生化反应筛选(SN标准)incubationmedia録三糖铁250用于副溶血性弧菌的生化反应筛选(SN标准)
SalmonellaShigiellaEnrichment
50161 BR 10  incubation media 50161 BR 10

50190 胰蛋白胨 BR 0g 酪蛋白胰酶水解而成的胨,培养基原材料  incubation media 50190 胰蛋白胨 BR 0g 酪蛋白胰酶水解而成的胨,培养基原材料
050190A (胰酪胨) BR 10  incubation media 050190A (胰酪胨) BR 10
50192 胰蛋白胨(进口) BR 500g 酪蛋白胰酶水解而成的胨,培养基原材料  incubation media 50192 胰蛋白胨(进口) BR 500g 酪蛋白胰酶水解而成的胨,培养基原材料
低密度脂蛋白受体相关蛋白9抗体规格四环素检定、厌氧亚盐还原杆菌、嗜热菌芽孢检验培养基

改良GAM琼脂抑菌剂 1ml*5 加入HB8462改良GAM琼脂中
匹克 匹克氏肉汤基础A Picks Broth BaseA 100 用于溶血性链球菌的增菌培养 (GB标准)
SBG磺胺增菌液250g/瓶含磺胺嘧啶,用于沙门氏菌选择性增菌incubationmediaSBG磺胺增菌液250g/瓶含磺胺嘧啶,用于沙门氏菌选择性增菌
CIN-1琼脂平板(9cm) 10/ 用于分离小肠结肠炎耶尔森氏菌
 

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