S期激酶相关蛋白1抗体图片

S期激酶相关蛋白1抗体图片

价格: 询价

品牌:上海博湖

货号:BH-K22696

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抗体名 :S期激酶相关蛋白1抗体图片

抗体英文名 :Anti-SKP1

靶点 :详见说明书

浓度 :1mg/1ml

应用范围 :WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

宿主 :详见说明书

供应商 :上海博湖

库存 :大量

级别 :详见说明书

目录编号 :详见说明书

抗原来源 :Rabbit

保质期 :详见说明书

适应物种 :详见说明书

标记物 :详见说明书

克隆性 :多克隆

保存条件 :Store at -20 °C

形态 :详见说明书

亚型 :IgG

免疫原 :KLH conjugated synthetic peptide derived from human SKP1

规格 :0.2ml/200μg


英文名称  Anti-SKP1
中文名称 S期激酶相关蛋白1抗体图片

     Cyclin A/CDK2 associated p19; Cyclin A/CDK2 associated protein p19; Cyclin-A/CDK2-associated protein p19; EMC 19; EMC19; OCP 2; OCP II; OCP II protein; OCP-2; OCP-II; OCP2; OCPII; organ of Corti protein 2; Organ of Corti protein II; p19A; p19skp1; RNA polymerase II elongation factor like protein; RNA polymerase II elongation factor like protein OCP2; RNA polymerase II elongation factor-like protein; S phase kinase associated protein 1; S phase kinase associated protein 1A; S phase kinase associated protein 1A p19A; S-phase kinase-associated protein 1; SIII; Skp 1; SKP 1A; skp1; SKP1_HUMAN; SKP1A; TCEB1L; Transcription elongation factor B (SIII) polypeptide 1 like; Transcription Elongation Factor B 1 like; Transcription Elongation Factor B 1-like; Transcription elongation factor B; Transcription elongation factor B SIII polypeptide 1 like. 
S期激酶相关蛋白1抗体图片      1mg/1ml
 0.2ml/200μg
抗体来源  Rabbit
克隆类型   polyclonal
交叉反应  Human, Mouse, Rat, Sheep
产品类型    一抗  
研究领域    细胞生物 免疫学
蛋白分子量  predicted molecular weight: 19kDa
     Lyophilized or Liquid
  KLH conjugated synthetic peptide derived from human SKP1
     IgG
纯化方法   affinity purified by Protein A
  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4


S期激酶相关蛋白1抗体图片产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.

Function : Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110.
Subunit : Component of an E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Component of the SCF(BTRC) complex, composed of SKP1, CUL1 and BTRC. Part of a SCF(BTRC)-like complex lacking CUL1, which is associated with phosphorylated NFKBIA and RELA; RELA interacts directly with NFKBIA. Component of the SCF(FBXO44) complex, composed of SKP1, CUL1 and FBXO44. Identified in a complex with SKP2, CKS1B and CDKN1B. Interacts with the cyclin A/CDK2 complex. Part of a SCF-like complex consisting of CUL7, RBX1, SKP1 and FBXW8. Part of several SCF complexes containing SKP1 and one of the F-box proteins. Component of a SCF(SKP2)-like complex containing CUL1, SKP1, TRIM21 and SKP2. Interacts with FBXO2, FBXO4, FBXW7 and TRIM21. Interacts with FBXO45 (By similarity). Component of the SCF(FBXO17) complex, composed of SKP1, CUL1 and FBXO17. Component of the SCF(FBXO27) complex, composed of SKP1, CUL1 and FBXO27. Component of the SCF(Cyclin F) complex consisting of CUL1, RBX1, SKP1 and CCNF.
Similarity : Belongs to the SKP1 family.
Database links : UniProtKB/Swiss-Prot: P63208.2


S期激酶相关蛋白1抗体图片抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,ElisaWB,免疫荧光等实验。
S期激酶相关蛋白1抗体图片实验原理 :
1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
2)活补体:IgMIgG1IgG2IgG3可通过经典途径激活补体,凝聚的IgAIgG4IgE可通过替代途径激活补体。
3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
4)可通过胎盘及粘膜:免疫球蛋白GIgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白AIgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,6070即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。


Anti-TRAF3/CDbp /FITC 荧光素标记CD结合蛋白/CD受体相关因子1抗体IgGMulti-class antibodies规格: 0.2ml
PTP-1B (Protein-tyrosine phosphatase, non-receptor ) 蛋白酪酸酶-1B(抗原)Multi-class antibodies规格: 0.5mg
转甲状腺素蛋白抗体 Anti-Transthyretin 0.2ml
14-3-3 family protein 英文名称: 苹果14-3-3蛋白抗体(植物) 1ml
EIF5 英文名称: 真核翻译起始因子5抗体 0.2ml
Rhesus antibody Rh phospho-IRS1(Thr176) 化胰岛素受体底物1抗体 规格 0.1ml
PTP-1B (Protein-tyrosine phosphatase, non-receptor ) 蛋白酪酸酶-1B(抗原)Multi-class antibodies规格: 0.5mg
S100B S100B蛋白抗原Multi-class antibodies规格: 0.5ml

Anti-Bcl-10/CLAP Bcl-10抗体Multi-class antibodies规格: 0.2ml
Rhesus antibody Rh PHGDH 甘油酸脱酶抗体 规格 0.2ml
IGF-1R 浓缩液 0.1ml 进口分装
URG4 英文名称: 上调基因4抗体(N) 0.2ml
C16orf72 英文名称: 16号染色体开放阅读框72抗体 0.1ml
Anti-Bcl-10/CLAP Bcl-10抗体Multi-class antibodies规格: 0.2ml
phospho-STXBP1 (Ser515) 英文名称: 化神经突触前膜胞内蛋白抗体 0.1ml

phospho-Ephrin B (Ser300) 英文名称: Ephrin B抗体 0.1ml
微管相关蛋白抗体 Anti-Tau protein 0.1ml
Anti-TIF1 beta/KAP1/FITC 荧光素标记转录中介因子Tif1β抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-LLGL1 + LLGL2(Ser650+Ser654) 化相似抑制基因HUGL抗体 规格 0.1ml
NOS-3 (eNOS) endotheli cell NOS 合成酶-3(抗原)Multi-class antibodies规格: 0.5mg
TrypticaseSoyBroth

甘露醇发酵培养基 250(g) incubation media 甘露醇发酵培养基 250(g)
科玛嘉李斯特氏菌显色培养基 1000ml/
其它菌检测培养基incubationmedia其它菌检测培养基
TSNAgar
SIMMONS citrate agar simmons柠檬酸盐琼脂 1.02501.0500 MERCK默克  incubation media SIMMONS citrate agar simmons柠檬酸盐琼脂 1.02501.0500 MERCK默克

Sodium-chloride peptone broth(buffered) 蛋白胨肉汤(缓冲) 1.10582.0500 MERCK默克  incubation media Sodium-chloride peptone broth(buffered) 蛋白胨肉汤(缓冲) 1.10582.0500 MERCK默克
Sorbitol-MacCONKEY agar(SMAC agar)SMAC琼脂/山梨醇麦康凯琼脂 1.09207.0500 MERCK默克  incubation media Sorbitol-MacCONKEY agar(SMAC agar)SMAC琼脂/山梨醇麦康凯琼脂 1.09207.0500 MERCK默克
SPS agar ANGELOTTI perfringens selective agar SPS琼脂/ANGELOTTI 1.10235.0500 MERCK默克  incubation media SPS agar ANGELOTTI perfringens selective agar SPS琼脂/ANGELOTTI 1.10235.0500 MERCK默克
S期激酶相关蛋白1抗体图片EGMedium

GN增菌液 Gram Negative Enrichment Broth 主要用于志贺氏菌的增菌培养,亦可用于沙门氏菌增菌培养(GB标准)
HAT混合盐 HAT Media Supplement (50×) Hybri-Max? 1vail γ射线灭菌
PYG液体培养基250g/瓶用于双歧杆菌乙酸和乳酸代谢物测定,每培养基中添加302incubationmediaPYG液体培养基250g/瓶用于双歧杆菌乙酸和乳酸代谢物测定,每培养基中添加3021319
m-EndoAgarLES
 

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