纺锤体和着丝粒相关蛋白2抗体规格

纺锤体和着丝粒相关蛋白2抗体规格

价格: 询价

品牌:上海博湖

货号:BH-K22677

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抗体名 :纺锤体和着丝粒相关蛋白2抗体规格

抗体英文名 :Anti-SKA2

靶点 :详见说明书

浓度 :1mg/1ml

应用范围 :WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

宿主 :详见说明书

供应商 :上海博湖

库存 :大量

级别 :详见说明书

目录编号 :详见说明书

抗原来源 :Rabbit

保质期 :详见说明书

适应物种 :详见说明书

标记物 :详见说明书

克隆性 :多克隆

保存条件 :Store at -20 °C

形态 :详见说明书

亚型 :IgG

免疫原 :KLH conjugated synthetic peptide derived from human SKA2

规格 :0.2ml/200μg


英文名称  Anti-SKA2
中文名称 纺锤体和着丝粒相关蛋白2抗体规格

     FAM33A; Family with sequence similarity 33, member A; FLJ12758; MGC110975; Protein FAM33A; SKA 2; SKA2; SKA2_HUMAN; Spindle and kinetochore associated complex subunit 2; Spindle and kinetochore associated protein 2; Spindle and kinetochore-associated protein 2; Spindle and KT (kinetochore) associated 2; Spindle and KT associated 2. 
纺锤体和着丝粒相关蛋白2抗体规格      1mg/1ml
 0.2ml/200μg
抗体来源  Rabbit
克隆类型   polyclonal
交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit
产品类型    一抗  
研究领域    细胞生物 细胞周期蛋白 细胞分化
蛋白分子量  predicted molecular weight: 14kDa
     Lyophilized or Liquid
  KLH conjugated synthetic peptide derived from human SKA2
     IgG
纯化方法   affinity purified by Protein A
  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4


纺锤体和着丝粒相关蛋白2抗体规格产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复) 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 Ska2 (spindle and kinetochore associated complex subunit 2), also known as FAM33A, is a 121 amino acid component of the Ska1 complex, a microtubule-binding subcomplex of the outer kinetochore that is critical for proper chromosome segregation. The Ska1 complex is a component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. Localized to the outer kinetochore and spindle microtubules during cell proliferation, Ska2 is essential for spindle checkpoint silencing and exit from mitosis. Downregulation of Ska2 leads to delayed recruitment of MAD2, a component of the mitotic spindle checkpoint, to several kinetochores resulting in occasional loss of individual chromosomes from the metaphase plate. Ska2 is encoded by a gene located on human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

Function : Component of the SKA1 complex, a microtubule-bindingsubcomplex of the outer kinetochore that is essential for properchromosome segregation. Required for timely anaphase onset duringmitosis, when chromosomes undergo bipolar attachment on spindlemicrotubules leading to silencing of the spindle checkpoint. TheSKA1 complex is a direct component of the kinetochore-microtubuleinterface and directly associates with microtubules as oligomericassemblies. The complex facilitates the processive movement ofmicrospheres along a microtubule in a depolymerization-coupledmanner. In the complex, it is required for SKA1 localization.
Subunit : Component of the SKA1 complex, composed of SKA1, SKA2 andSKA3. Forms a heterodimer with SKA1; the heterodimer interactingwith SKA3. The core SKA1 complex is composed of 2 SKA1-SKA2heterodimers, each heterodimer interacting with a molecule of theSKA3 homodimer. The core SKA1 complex associates with microtubulesand forms oligomeric assemblies. Interacts directly with SKA1.Binds directly to microtubules; but with a much lower affinity thanSKA1. May interact with NR3C1; the relevance of such interactionremains unclear in vivo.
Subcellular Location : Cytoplasm, cytoskeleton, spindle.Chromosome, centromere, kinetochore. Note=Localizes to the outerkinetochore and spindle microtubules during mitosis in a NDC80complex-dependent manner. Localizes to both the mitotic spindle andkinetochore-associated proteins.
Similarity : Belongs to the SKA2 family.
Database links : UniProtKB/Swiss-Prot: Q8WVK7.1


纺锤体和着丝粒相关蛋白2抗体规格抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,ElisaWB,免疫荧光等实验。
纺锤体和着丝粒相关蛋白2抗体规格实验原理 :
1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
2)活补体:IgMIgG1IgG2IgG3可通过经典途径激活补体,凝聚的IgAIgG4IgE可通过替代途径激活补体。
3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
4)可通过胎盘及粘膜:免疫球蛋白GIgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白AIgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,6070即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。


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哥伦比亚CNA琼脂 100(g) incubation media 哥伦比亚CNA琼脂 100(g)
七叶苷发酵管 七叶苷发酵管 20 BR
XLD琼脂250选择性培养基,用于志贺氏菌的选择性培养(FDA标准)incubationmediaXLD琼脂250选择性培养基,用于志贺氏菌的选择性培养(FDA标准)
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KLIGLER agar Double sugar iron agar acc.toKLIGLER KLIGLER 1.03913.0500 MERCK默克  incubation media KLIGLER agar Double sugar iron agar acc.toKLIGLER KLIGLER 1.03913.0500 MERCK默克

双糖铁琼脂  incubation media 双糖铁琼脂
Lactose broth 乳糖肉汤 1.07661.0500 MERCK默克  incubation media Lactose broth 乳糖肉汤 1.07661.0500 MERCK默克
AgarLactose TTC with Tergitol( base) 乳糖TTC琼脂 1.07680.0500 MERCK默克  incubation media AgarLactose TTC with Tergitol( base) 乳糖TTC琼脂 1.07680.0500 MERCK默克
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Pick’sBrothBaseA
GVPCLiquidMedium
 

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