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库存 :20
细胞类型 :I类
品系 :优良品
组织来源 :ATCC来源
物种来源 :ATCC来源
细胞形态 :贴壁
器官来源 :ATCC来源
运输方式 :常温运输或冻存干冰运输
年限 :3~5代
生长状态 :优良
规格 :5×106cells/T25细胞培养瓶
Synonyms: | KHOS/NP(人骨肉瘤细胞) |
Background: | This cell line was derived from HOS (ATCC CRL-1543) by transformation using Kirsten murine sarcoma virus (Ki-MSV). |
Species: | Homo sapiens, human |
Tissue: | bone |
Disease: | osteosarcoma |
Gender: | female, 13 years, Caucasian |
Growth Mode: | adherent |
DNA Profile: | Amelogenin: X CSF1PO: 12 D13S317: 12 D16S539: 10,13 D5S818: 13 D7S820: 11,12 THO1: 6 TPOX: 8,11 vWA: 18 Cell Line Authentication Service |
Culture Medium: | MEM + 10% FBS+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) We strongly suggest to purchase the complete medium |
Cryopreservation medium: | 90%FBS+10%DMSO |
Tumor Formation: | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
Comments: | The cells exhibit a high saturation density, a high plating efficiency in soft agar and produce tumors in nude mice. Cells carry the Ki-MSV genome but do not produce infectious virus particles or viral antigens. |
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