转化生长因子β1/TGF β1/TGF-β1抗体品牌

抗体名 ：转化生长因子β1/TGF β1/TGF-β1抗体品牌

抗体英文名 ：Anti-TGF beta 1

靶点 ：详见说明书

浓度 ：1mg/1ml

应用范围 ：WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

宿主 ：详见说明书

供应商 ：上海博湖

库存 ：大量

级别 ：详见说明书

目录编号 ：详见说明书

抗原来源 ：Rabbit

保质期 ：详见说明书

适应物种 ：详见说明书

标记物 ：详见说明书

克隆性 ：多克隆

保存条件 ：Store at -20 °C

形态 ：详见说明书

亚型 ：IgG

免疫原 ：KLH conjugated synthetic peptide derived from human TGF-Beta 1

规格 ：0.2ml/200μg

英文名称  Anti-TGF beta 1
中文名称 转化生长因子β1/TGF β1/TGF-β1抗体品牌
别    名  CED; DPD1; LAP; Latency-associated peptide; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1. 
转化生长因子β1/TGF β1/TGF-β1抗体品牌浓    度  1mg/1ml
规 格  0.1ml/100μg 0.2ml/200μg
抗体来源  Rabbit
克隆类型   polyclonal
交叉反应  Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig
产品类型    一抗  
研究领域    心血管 信号转导 生长因子和激素 转录调节因子
蛋白分子量  predicted molecular weight: 12.8kDa
性    状  Lyophilized or Liquid
免 疫 原   KLH conjugated synthetic peptide derived from human TGF-Beta 1
亚    型  IgG
纯化方法   affinity purified by Protein A
储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

转化生长因子β1/TGF β1/TGF-β1抗体品牌产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
Function : Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
Subunit : Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN.
Subcellular Location : Secreted, extracellular space, extracellular matrix.
Tissue Specificity : Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
Post-translational modifications : Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
DISEASE : Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Similarity : Belongs to the TGF-beta family.
Database links :
UniProtKB/Swiss-Prot: P01137.2
Entrez Gene: 7040 Human
Entrez Gene: 21803 Mouse
Entrez Gene: 59086 Rat
Omim: 190180 Human
SwissProt: P01137 Human
SwissProt: P04202 Mouse
SwissProt: P17246 Rat
Unigene: 645227 Human
生长因子和激素（ Growth Factor and Hormones） TGF是一种多效生长因子，对上皮细胞增值有潜在抑制作用，可抑制肿瘤生长用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。 TGFβ超级家族由为数众多的生长的分化因子组成，包括转移生长因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盘生长因子(PTGF-β)；生长/分化因子(GDFs)；缪氏抑制物(MIS)；骨形态形成蛋白(BMPs)；交织细胞元神经生长因子(GDNF)；抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超级家族成员参与胚胎发育和成体组织体内平衡。TGF-β1抗体与TGF-β2和TGF-β3没有交叉反应。与豚鼠有大部分交叉。


转化生长因子β1/TGF β1/TGF-β1抗体品牌抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体 抗体的交叉反应： 人，小鼠，大鼠，鸡 ，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。
转化生长因子β1/TGF β1/TGF-β1抗体品牌实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。

Anti-Tyk2/Non receptor tyrosine protein kinase 2/FITC 荧光素标记非受体酪酸蛋白激酶2抗体IgGMulti-class antibodies规格： 0.2ml
CCL14/HCC-1(C-C motif chemokine 14) 嗜酸粒细胞趋化蛋白14抗原Multi-class antibodies规格： 0.5mg
细胞质膜微囊蛋白-1抗体 Anti-Cav-1 0.1ml
SEMA6C 英文名称： 轴突导向因子SEMA6C抗体 0.2ml
ECG2 英文名称： 食道癌相关基因抗体 0.1ml
Rhesus antibody Rh Phospho-HP1(Tyr43) 异染色质蛋白1化抗体(果蝇) 规格 1ml
CCL14/HCC-1(C-C motif chemokine 14) 嗜酸粒细胞趋化蛋白14抗原Multi-class antibodies规格： 0.5mg
mouse Caspase-2 小鼠Caspase-2Multi-class antibodies规格： 48T
Anti-FLIP S/L 凋亡调节基因之一抗体Multi-class antibodies规格： 0.1ml
Rhesus antibody Rh Mouse Anti-rabbit IgG/PE-Cy7 PE-Cy7标记的小鼠抗兔IgG 规格 0.1ml
Gal(Human Alpha-galactoyl) ELISA Kit 人α半乳糖基抗体 96T
Syntrophin-2 英文名称： 互养蛋白2抗体 0.1ml
CCDC83 英文名称： 卷曲螺旋结构域蛋白83抗体 0.1ml
Anti-FLIP S/L 凋亡调节基因之一抗体Multi-class antibodies规格： 0.1ml
Rabbit anti-mouse Kappa light chain/PE-CY5 PE-CY5标记的兔抗小鼠k链 0.1ml
Frizzled 2 英文名称： Wnt信号受体FZD2蛋白抗体 0.1ml
富半胱酸肝素结合蛋白61 anti-Cyr61 0.2ml
Anti-PAP2c /FITC 荧光素标记磷脂酸酶2C抗体IgGMulti-class antibodies规格： 0.2ml
Rhesus antibody Rh Phospho-Tuberin/TSC2(Ser664) 化结节性硬化蛋白抗体 规格 0.1ml
Goat Anti-bov IgM/FITC 荧光素标记羊抗IgMMulti-class antibodies规格： 0.3ml
HuTu-80(人十二脂肠腺癌) 5×106cells/瓶×2
大鼠腹脊髓神经元RN-vsc
CD226 Others Rat 大鼠 CD226 / DNAM-1 人细胞裂解液 (阳性对照)
JEG细胞，人绒毛膜癌 大鼠肝细胞,IAR20细胞 人心脏成纤维细胞-心房HCF-aa
CM-R057大鼠肾系膜细胞完全培养基100mL
769-P(人肾细胞腺癌细胞) 5×106cells/瓶×2 CM-H083人脐动脉内皮细胞完全培养基100mL 人间充质干细胞-骨髓RNAHMSC-bm miRNA5 μg
肺动脉成纤维细胞Many types of cells包装：5 × 105次方(1ml)
Spike Others MERS-CoV 中东呼吸综合征冠状病毒 MERS-CoV (HCoV-EMC/2012) Spike Protein S1 (aa 1-725) 人细胞裂解液 (阳性对照)
人结直肠腺癌细胞;Caco-2
293T细胞，SV永生化的人胚肾上皮细胞 蚊子细胞,C6/36细胞 CL-0250801-D (人巨细胞性肺癌细胞)5×106cells/瓶×2
ZR-75-1(人癌细胞) 5×106cells/瓶×2
HAoSMC-c 人主动脉平滑肌细胞(HAoSMC) 500,000cells 胰岛β细胞Many types of cells包装：5 × 105次方(1ml)
转化生长因子β1/TGF β1/TGF-β1抗体品牌HuTu-80(人十二脂肠腺癌) 5×106cells/瓶×2
大鼠腹脊髓神经元RN-vsc
CD226 Others Rat 大鼠 CD226 / DNAM-1 人细胞裂解液 (阳性对照)
JEG细胞，人绒毛膜癌 大鼠肝细胞,IAR20细胞 人心脏成纤维细胞-心房HCF-aa
CM-R057大鼠肾系膜细胞完全培养基100mL
769-P(人肾细胞腺癌细胞) 5×106cells/瓶×2 CM-H083人脐动脉内皮细胞完全培养基100mL 人间充质干细胞-骨髓RNAHMSC-bm miRNA5 μg