基底膜相关软骨素蛋白多糖抗体品牌

抗体名 ：基底膜相关软骨素蛋白多糖抗体品牌

抗体英文名 ：Anti-SMC3/CSPG6

靶点 ：详见说明书

浓度 ：1mg/1ml

应用范围 ：WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

宿主 ：详见说明书

供应商 ：上海博湖

库存 ：大量

级别 ：详见说明书

目录编号 ：详见说明书

抗原来源 ：Rabbit

保质期 ：详见说明书

适应物种 ：详见说明书

标记物 ：详见说明书

克隆性 ：多克隆

保存条件 ：Store at -20 °C

形态 ：详见说明书

亚型 ：IgG

免疫原 ：KLH conjugated synthetic peptide derived from human SMC3/CSPG6

规格 ：0.2ml/200μg

基底膜相关软骨素蛋白多糖抗体品牌Anti-SRC /FITC 荧光素标记整合素样金属蛋白酶与凝血酶1型-7抗体IgGMulti-class antibodies规格： 0.2ml
EXPB-2 植物延展素-β(抗原)Multi-class antibodies规格： 0.5mg
蛋白酶激活受体-1抗体 Anti-PAR-1 0.2ml
SERPINA12 英文名称： 内脏脂肪组织源性丝酸蛋白酶抑制蛋白抗体 0.2ml
Phospho-Ezrin (Tyr478) 英文名称： 化埃兹蛋白抗体 0.1ml
Rhesus antibody Rh phospho-NFKB p65(Thr254) 化细胞核因子抗体 规格 0.1ml
EXPB-2 植物延展素-β(抗原)Multi-class antibodies规格： 0.5mg
英文名称  Anti-SMC3/CSPG6
中文名称 基底膜相关软骨素蛋白多糖抗体品牌

别    名  BAM; Bamacan; Basement membrane associated chondroitin proteoglycan; Basement membrane-associated chondroitin proteoglycan; BMH; CDLS3; chondroitin sulfate proteoglycan 6 (bamacan); Chondroitin sulfate proteoglycan 6; Chromosome associated polypeptide; Chromosome-associated polypeptide; CSPG 6; CSPG6; hCAP; SMC 3; SMC protein 3; SMC-3; smc3; SMC3_HUMAN; SMC3L1; Structural maintenance of chromosome 3; Structural maintenance of chromosomes 3; Structural maintenance of chromosomes protein 3. 
基底膜相关软骨素蛋白多糖抗体品牌浓    度  1mg/1ml
规 格  0.2ml/200μg
抗体来源  Rabbit
克隆类型   polyclonal
交叉反应  Human, Mouse, Rat, Chicken, Dog, Cow, Rabbit
产品类型    一抗  
研究领域    肿瘤 细胞生物 免疫学 染色质和核信号 细胞周期蛋白 结合蛋白 细胞分化 表观遗传学
蛋白分子量  predicted molecular weight: 142kDa
性    状  Lyophilized or Liquid
免 疫 原   KLH conjugated synthetic peptide derived from human SMC3/CSPG6
亚    型  IgG
纯化方法   affinity purified by Protein A
储 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

基底膜相关软骨素蛋白多糖抗体品牌产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
Function : Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.
Subunit : Interacts with MXI1, MXD3 and MXD4. Interacts with SYCP2. Found in a complex with SMC1A, CDCA5 and RAD21, PDS5A/APRIN and PDS5B/SCC-112 (By similarity). Forms a heterodimer with SMC1A or SMC1B in cohesin complexes. Cohesin complexes are composed of the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer attached via their hinge domain, RAD21 which link them, and one STAG protein (STAG1, STAG2 or STAG3), which interacts with RAD21. Also found in meiosis-specific cohesin complexes. Interacts with NUMA1, and forms a ternary complex with KIF3B and KIFAP3, suggesting a function in tethering the chromosomes to the spindle pole and in chromosome movement. Interacts with PDS5A and WAPAL; regulated by SMC3 acetylation. Interacts with RPGR (By similarity).
Subcellular Location : Nucleus. Chromosome. Chromosome, centromere. Note=Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. The phosphorylated form at Ser-1083 is preferentially associated with unsynapsed chromosomal regions (By similarity).
Post-translational modifications : Phosphorylated at Ser-1083 in a SPO11-dependent manner (By similarity).
Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. Deacetylation by HDAC8, regulates release of the cohesin complex from chromatin.
DISEASE : Cornelia de Lange syndrome 3 (CDLS3) [MIM:610759]: A form of Cornelia de Lange syndrome, a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. Characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation, hirsutism, gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. Cornelia de Lange syndrome type 3 is a mild form with absence of major structural anomalies. The phenotype in some instances approaches that of apparently non-syndromic mental retardation. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity : Belongs to the SMC family. SMC3 subfamily.
Database links : UniProtKB/Swiss-Prot: Q9UQE7.2


基底膜相关软骨素蛋白多糖抗体品牌抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。 抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体 抗体的交叉反应： 人，小鼠，大鼠，鸡 ，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。

Anti-Phospho-PAK2 (Ser20) /FITC 荧光素标记化p21激活激酶2抗体IgGMulti-class antibodies规格： 0.2ml
Goat Anti-Chicken IgG 羊抗鸡IgG (亲和层析纯化)Multi-class antibodies规格： 1mg
抗g-基B2受体抗体 anti-GABAB2 0.1ml
Donkey Anti-Guinea pig IgG/HRP 辣根过氧化物酶标记的驴抗豚鼠IgG 0.1ml
TMA16 英文名称： 4号染色体开放阅读框43抗体 0.2ml
Rhesus antibody Rh phospho-Src(Tyr529) 化Src原癌基因抗体 规格 0.1ml
Goat Anti-Chicken IgG 羊抗鸡IgG (亲和层析纯化)Multi-class antibodies规格： 1mg
Rabbit anti-mouse Kappa Chain whole serum 兔抗小鼠k-链抗血清 1ml
Factor D 英文名称： 补体因子D抗体 0.2ml
白介素-17抗体 Anti-IL-17 0.1ml
Anti-pro-caspase3/FITC 荧光素标记半胱天冬酶-3酶原抗体IgGMulti-class antibodies规格： 0.2ml
Rhesus antibody Rh Phospho-PTPN11(Tyr542) 化蛋白酪酸酶2抗体 规格 0.1ml
Rabbit Anti-human IgGF(ab')2 Whole serum 兔抗人IgGF(ab')2抗血清Multi-class antibodies规格： 1ml
小鼠白介素27(IL-27)ELISAKit   ELISA.   96T/48T
小鼠白介素23(IL-23)ELISAKit   ELISA.   96T/48T
小鼠心肌营养素1(CT-1)ELISAKit   ELISA.   96T/48T
小鼠抗α-胞衬蛋白抗体IgG/IgA(α-FodrinIgG/IgA)ELISAKit   ELISA.   96T/48T
大鼠内皮素1(EDN1)ELISA试剂盒 ，英文名： EDN1 ELISA Kit
Mouse nicotinamide adenine dinucleotide phosphate (NADPH) ELISA Kit 小鼠烟酰胺腺嘌呤二核苷酸(NADPH)ELISA试剂盒
RatGlycogenphosphorylaseII,GP-IIELISAKit 大鼠糖原化酶同工酶II(GP-II)ELISA试剂盒 96T/48T 进口分装
CLIAKitforGIPELISAKit大鼠葡萄糖依赖性胰岛素释放多肽规格：48T/96T
细胞脯酰肽酶(prolinase)比色法定量检测试剂盒20次
RatsolubleP-selectin,sP-selectinELISAKit大鼠可溶性P选择素(sP-selectin)ELISA试剂盒规格：96T/48T
基底膜相关软骨素蛋白多糖抗体品牌小鼠过氧化物酶体生物合成因子2(PEX2)ELISA试剂盒 ，英文名： PEX2 ELISA Kit
人磷酯酶C(PLC)ELISA检测试剂盒HumanPhospholipaseC,PLCELISAKit 96T/48T
大鼠糖缺失性转铁蛋白(CDT)免疫试剂盒 Rat carbohydrate-deficie ansferrin,CDT ELISA Kit
英文名称RatAquaporin3ELISAKit大鼠水通道蛋白3(AQP3)规格：96T/48T
化妆品糖精(saccharin)含量比色法定量检测试剂盒20次
ELISAKitANG-Ⅱ人血管紧张素Ⅱ规格：48T/96T
基底膜相关软骨素蛋白多糖抗体品牌实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。