文献支持人小细胞肺癌细胞（STR鉴定）

注册证号 ：科研

英文名 ：NCI-H446

CAS号 ：/

库存 ：10

供应商 ：上海烜雅生物科技有限公司

肿瘤类型 ：人小细胞肺癌细胞（STR鉴定）

细胞类型 ：科研

ATCC Number ：/

品系 ：人小细胞肺癌细胞（STR鉴定）

组织来源 ：人小细胞肺癌细胞（STR鉴定）

相关疾病 ：人小细胞肺癌细胞（STR鉴定）

物种来源 ：鼠/人/其它

免疫类型 ：人小细胞肺癌细胞（STR鉴定）

细胞形态 ：上皮细胞样 贴壁生长 少量悬浮

是否是肿瘤细胞 ：是

器官来源 ：肺；转移灶：肋膜渗出癌；小细胞肺癌

运输方式 ：常温运输/干冰运输

年限 ：尽快解冻复苏细胞进行培养

生长状态 ：贴壁/悬浮

规格 ：1x10^6

人小细胞肺癌细胞（STR鉴定）购买须知：
产品简介：
[品系] ……………………Human
[组织来源]………………详情请咨询
[生长状态]………………贴壁/悬浮
[细胞类型]………………详情请咨询
[疾病] ……………………正常
[应用] ……………………科研
NCI-H446细胞株从一位小细胞肺癌患者的胸水中建立。细胞的原始形态并不具有小细胞肺癌特征。这个细胞株是小细胞肺癌的生化和形态学上的变种，表达神经元特有的烯醇酶和脑部肌酸激酶同功酶。左旋多巴脱羧酶、蚕素、抗利尿激素、催产素或胃泌激素释放肽未达到可检测水平。C-myc DNA序列扩增约20倍，c-myc RNA比正常细胞增加15倍。最初传代培养基用添加10 nM 氢化可的松, 0.005 mg/ml 胰岛素, 0.01 mg/ml 铁传递蛋白, 10 nM 17-beta-雌二醇,30 nM ya硒 钠的RPMI 1640, 95%，胎牛血清, 5%。
生物安全：
不含有 HIV-1、 HBV、HCV、支原体、细菌、酵母和真菌等。
产品包装：
提供新鲜或者冻存的细胞
使用方法：
如是新鲜细胞，客户收到细胞后应立即将其放入CO2细胞培养箱内静置3-4个小时，再进行后续的实验操作；如是冻存细胞， 客户收到细胞后应立即将其放入液氮、-80℃冰箱或立即进行复苏。

细胞培养 ：
（1）Getting Started with an ATCC Cell Line：
ATCC cell lines and hybridomas are shipped frozen on dry ice in cryopreservation vials or as growing
cultures in flasks at ambient temperature. Upon receipt of frozen cells, it is important to immediately revive
them by thawing and removing the DMSO and placing them into culture. If this is not possible, store the
cells in liquid nitrogen vapor (below −130°C). Do not store frozen cells at temperatures above −130°C as
their viability will decline rapidly.

（2）细胞图片：


（3）Initiating Frozen Cultures：
1. Prepare a culture vessel so that it contains the recommended volume
of the appropriate culture medium as listed on the Product Sheet,
equilibrated for temperature and pH (CO₂).
2. Thaw the vial by gentle agitation in a water bath at 37°C or the normal growth temperature for that cell
line. Thawing should be rapid, approximately 2 minutes or until ice crystals have melted.
3. Remove the vial from the water bath and decontaminate it by dipping in or spraying with 70% ethanol.
Follow strict aseptic conditions in a laminar flow tissue culture hood for all further manipulations.
4. Unscrew the top of the vial and transfer the contents to a sterile
centrifuge tube containing 9 mL of the recommended medium.
Remove the cryoprotectant agent (DMSO) by gentle centrifugation (10
minutes at 125 × g). Discard the supernatant, and resuspend the cells
in 1 or 2 mL of complete growth medium. Transfer the cell suspension
into the culture vessel containing the complete growth medium and
mix thoroughly by gentle rocking. （4）Processing Flask Cultures：
Some ATCC cell, are shipped as growing cultures in culture vessels. These vessels are seeded with cells,
incubated to ensure cell growth and then filled completely with medium for shipping.
Upon receiving a flask culture, visually examine the medium for macroscopic evidence of microbial contamination. This includes
unusual pH shifts (yellow or purple color from the phenol red),
turbidity, or particles. With an inverted microscope at low power
(100×) check the medium for evidence of microbial contamination
as well as the morphology of the cells. See page 6 for more details
on examining cell cultures.
烜雅生物发布
质量可靠，售后有保障
如运输过程中导致细胞污染或者死亡，我们将无条件补发收货后十个工作日内有其他问题提供照片可半价重发
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