SpCas9 Nuclease，SpCas9核酸酶，用于基因编辑的Cas9蛋白

价格： ¥2451

品牌：法国Polyplus Transfection

货号：722-100

产品详情

jetCRISPR™

RNP transfection reagent for genome editing

Specifically designed for Cas protein (Cas9, Cpf1) and guide RNA delivery
High genome editing efficiency
Excellent cell viability and morphology
Fast and reliable gene editing
Easy to use: Reverse and Forward protocols

Specifications

Reagent	jetCRISPR™
Molecule delivered	Ribonucleoprotein: Guide RNA and Cas protein (Cas9, Cpf1)
Application	CRISPR mediated Genome editing
Cell types	Adherent and suspension cells
Number of transfections	1.5 ml of jetCRISPR™ transfection reagent is sufficient to perform up to 1250 transfections in 24-well plates or 300 transfections in 6-well plates
Storage	5°C ± 3°C, stable for 6 months (502-01) to at least one year (other packaging sizes) when stored appropriately

Summary

CRISPR/Cas engineered nuclease system is a powerful and highly specific genome editing tool. CRISPR/Cas is a two component system with a guide RNA (gRNA) molecule that drives a Cas nuclease (Cas9, Cpf1) to a specific targeted sequence within the genome in order to introduce genetic modifications (mutations, insertions or deletions).

jetCRISPR™ is an innovative reagent especially designed to deliver RNP in a CRISPR/Cas9 and CRISPR/Cpf1 experiments. jetCRISPR™ leads to high CRISPR genome editing efficiency, while maintaining excellent cell viability and morphology post-transfection. Use the leading technology for CRISPR/Cas9 and CRISPR/Cpf1 RNP delivery!

Increase your CRISPR/Cas9 genome editing efficiency using SpCas9 Nuclease!

Ordering information

Product	Reference Number	Product size
jetCRISPR™	502-01	0.1 ml
jetCRISPR™	502-07	0.75 ml
jetCRISPR™	502-15	1.5 ml
SpCas9 Nuclease	722-100	100 µg

High Genome Editing efficiency

jetCRISPR™ has been specifically designed for high transfection efficiency of RNP (guide RNA and Cas9 protein complex), thus leading to a high cas9-mediated genome editing efficiency on a wide variety of targets in different cell types (Fig. 1 & 2).

Fig. 1: High genome editing efficiency using jetCRISPR™ in HeLa cells. RNP transfections were performed in HeLa cells using several RNP concentrations of SpCas9 Nuclease and HPRT1 sgRNA or negative control in combination with 0.3 µl of jetCRISPR™ reagent per well of a 96-well plate. At 48 h post-transfection, T7 digestion products were run on a 2% agarose gel and stained with BET displayed by G:Box transilluminator (Syngene^®). Acquisitions were carried out with the Genesnap software (Syngene^®) and INDEL quantifications were performed with the Genetools software (Syngene^®). 1: Uncleaved fragment of 1083 bp, 2: long cleaved fragment of 827 bp, 3: short cleaved fragment of 256 bp.

jetCRISPR™ transfection reagent achieves higher genome editing efficiency than other competitors, which makes it the product of choice for CRISPR applications.

jetCRISPR - Comparison of genome editing efficiency

Fig. 2: Superior genome editing efficiency obtained with jetCRISPR™ in comparison with Lipofectamine^® CRISPRMAX™. RNP transfections were performed in A549 and HEK-293 cells using 30 nM RNP (SpCas9 Nuclease and HPRT1 sgRNA) with 0.3 µl of jetCRISPR™ reagent or 0.3 µl of Lipofectamine^® CRISPRMAX™, per well of a 96-well plate. At 48 h post-transfection, genome editing was assessed by calculating the percentage (%) of INDEL using the T7 endonuclease method. The INDEL % was determined by using Genetools software (Syngene^®).