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抗体名 ：NRP1 Protein
抗体英文名 ：NRP1 Protein
宿主 ：兔
供应商 ：上海研卉生物科技有限公司
库存 ：大量
抗原来源 ：其它
是否单克隆 ：0
规格 ：50ug
【产品介绍】Neuropilin- 1（NRP1）是细胞表面相对分子质量为103kDa的Ⅰ型跨膜糖蛋白。它有两种亚型：亚型1包括蛋白全长，有923个氨基酸组成，是结合在膜上的糖蛋 白；亚型2经过剪切后只有644个氨基酸组成，是可溶的分泌性蛋白。研究表明，NRP1通常表达在内皮细胞及一些肿瘤细胞，是 Semaphorin3A（Sema3A）和血管内皮生长因子165（vascular endothelial growth factor165，VEGF165）的受体，在神经轴突的生长、血管的生成、发育和肿瘤转移中起着重要的作用。NRP1 Protein，NRP1蛋白
NRP1 Fusion Protein
Catalog number: Ag23549
Product Name:
human NRP1-6*His fusion protein
Catalog No:
Ag23549
Source:
e coli.-derived, PET28a
Peptide Sequence:
EWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIK
(483-644 aa encoded by BC007533)
(483-644 aa encoded by BC007533)
Activity:
Not tested.
Endotoxin Level:
Please contact the lab for more information.
Note：Remove endotoxin and sterilization is required for cell assay.
Note：Remove endotoxin and sterilization is required for cell assay.
Purity:
90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
Formulation:
The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
Reconstitution and Storage
Reconstitution:
Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
Shipping:
The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).
Stability and Storage:
Store for up to 12 months at -20°C to -80°C as lyophilized powder.
Storage of Reconstituted Protein:
Short-term storage: Store at 2-8°C for (1-2 weeks).
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
上海研卉生物科技有限公司
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入驻年限：10年
