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抗体英文名 :BMP2
宿主 :兔
库存 :大量
抗原来源 :其它
供应商 :上海研卉生物科技有限公司
是否单克隆 :多克隆
规格 :10μg
【产品介绍】骨形态发生蛋白-2(Bone m o r phogenetic protein 2, BMP-2)是转化生长因子β超家族成员之一,是一种分泌性多功能蛋白。目前,已经发现的该超家族成员约有10余种,BMPs合成时是一个大的前体蛋白, 包括一个信号肽、一个前结构域和一个羧基末端区。羧基末端区约含有100-125个氨基酸,该区域从前体蛋白上水解被释放形成二聚体,释放至细胞外与机体 各处靶细胞表面的相应受体结合而发挥作用。具有诱导未分化的间充质干细胞向成软骨细胞和成骨细胞定向分化与增殖的能力,促进成骨细胞分化成熟,参与骨和软 骨的生长发育及其重建过程,进而加速骨缺损的修复。Recombinant Human/Murine/Rat BMP-2 (E.coli derived)
Product Details
BMPs (Bone Morphogenetic Proteins) belong to the TGF-β superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis, and is expressed in a variety of tissues, including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26 kDa protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant Human/Murine/Rat BMP-2 is a 26.0 kDa homodimeric protein consisting of two 115 amino acid polypeptide chains.
Source: E.coli
Synonyms: Bone Morphogenetic Protein-2, BMP-2A
AA Sequence (monomer): MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.0 μg/ml.
Product Details
Source: Polyclonal Rabbit
Preparation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hBMP-2. Anti-Human BMP-2 specific antibody was purified by affinity chromatography employing immobilized hBMP-2 matrix.
Immunogen: E.coli derived Recombinant Human/Murine/Rat BMP-2 (PeproTech catalog# 120-02)
Sandwich ELISA: To detect hBMP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech’s Biotinylated Anti-Human BMP-2 (500-P195Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hBMP-2.
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入驻年限:10年