磷酸化精子尾部结构蛋白抗体图片

磷酸化精子尾部结构蛋白抗体图片

价格: 询价

品牌:上海博湖

货号:BH-K20432

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抗体名 :磷酸化精子尾部结构蛋白抗体图片

抗体英文名 :Anti-phospho-ODF2(Ser796)

靶点 :详见说明书

浓度 :1mg/1ml

应用范围 :WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

宿主 :详见说明书

供应商 :上海博湖

库存 :大量

级别 :详见说明书

目录编号 :详见说明书

抗原来源 :Rabbit

保质期 :详见说明书

适应物种 :详见说明书

标记物 :详见说明书

克隆性 :多克隆

保存条件 :Store at -20 °C

形态 :大量

亚型 :IgG

免疫原 :KLH conjugated synthesised phosphopeptide derived from human ODF2 around the phosphorylation site of Ser796

规格 :0.2ml/200μg


英文名称  Anti-phospho-ODF2(Ser796)
中文名称 磷酸化精子尾部结构蛋白抗体图片

     ODF2(phospho S796);84 kDa outer dense fiber protein; Cancer/testis antigen 134; Cenexin 1; Cenexin; Cenexin1 variant 1; CT134; FLJ44866; KKT 4; KKT4; MGC111096; MGC9034; ODF 2; ODF 84; ODF2; ODF2/1; ODF2/2; ODF84; OTTHUMP00000022274; OTTHUMP00000022275; Outer dense fiber of sperm tail 2; Outer dense fiber of sperm tails 2; outer dense fiber of sperm tails; Outer dense fiber of sperm tails protein 2; Outer dense fiber of sperm tails, 84 kD; Outer dense fiber protein 2; Sperm outer dense fiber major protein 2; sperm tail structural protein; Sperm tail structural protein; Testis specific autoantigen. 
磷酸化精子尾部结构蛋白抗体图片    
 1mg/1ml
 0.1ml/100μg
抗体来源
 Rabbit
克隆类型
  polyclonal
交叉反应
 Human, Pig
产品类型    一抗 磷酸化抗体
 
研究领域    细胞生物 免疫学 发育生物学 染色质和核信号 信号转导 细胞周期蛋白 细胞分化 表观遗传学

蛋白分子量  predicted molecular weight: 93kDa
   
 Lyophilized or Liquid
  KLH conjugated synthesised phosphopeptide derived from human ODF2 around the phosphorylation site of Ser796
   
 IgG
纯化方法
  affinity purified by Protein A
  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化精子尾部结构蛋白抗体图片产品应用  WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  

保存条件
 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品介绍 Cenexin1 is an isoform of ODF2, that unlike ODF2 is present in several somatic cell types. Cenexin1 acts as a general scaffold protein that is specifically localised to the distal/subdistal appendages of mother centrioles. Cenexin1 is required for proper localization of Plk1 to the centrosomes. This centrosomal localization of Plk1 is required for proper microtubule function. Cenexin1 recruits Plk1 via a C-terminal extension of cenexin1 that is not present in ODF2. Cenexin1 is required for proper mitotic progression; depletion of Cenexin1 ultimately leads to chromosome missegregation and apoptosis. The ODF2 (outer dense fiber 2) gene encodes both ODF2 and Cenexin1, which have very different functions. ODF2 is a major component of sperm tail outer dense fibers (ODFs). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail. They may help to maintain the passive elastic structures and elastic recoil of the sperm tail, and may also modulate sperm motility.

Function : Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly.
Subunit : Self-associates. Associates with microtubules and forms a fibrillar structure partially linked to the microtubule network. Interacts via its C-terminus with PLK1. Interacts with ODF1 (By similarity).
Subcellular Location : Cytoplasm, cytoskeleton, centrosome. Cell projection, cilium. Cytoplasm, cytoskeleton, centrosome, centriole. Cytoplasm, cytoskeleton, spindle pole. Note=Localized at the microtubule organizing centers in interphase and spindle poles in mitosis. Localized at the distal/subdistal appendages of mother centrioles.
Tissue Specificity : Testis-specific (at protein level). Highly expressed in cytoplasm of step 2 round spermatids. Detected in the middle piece and extends to about half the principal piece of the sperm tails.
Post-translational modifications : Tyrosine phosphorylated (By similarity).
Similarity : Belongs to the ODF2 family.
Database links : NCBI Reference Sequence: NP_002531.3


磷酸化精子尾部结构蛋白抗体图片抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。 
如果所用抗原浓度IC50浓度为pg/,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。

3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml 抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体 抗体的交叉反应: 人,小鼠,大鼠,鸡 ,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,ElisaWB,免疫荧光等实验。
磷酸化精子尾部结构蛋白抗体图片实验原理 :
1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。

2)活补体:IgMIgG1IgG2IgG3可通过经典途径激活补体,凝聚的IgAIgG4IgE可通过替代途径激活补体。
3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
4)可通过胎盘及粘膜:免疫球蛋白GIgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白AIgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,6070即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。

肿瘤坏死因子受体超家族成员13B抗体  Anti-TNFRSF13B  WB IHC-P IHC-F IF  100ul
内质网蛋白44抗体  Anti-TXNDC4  WB IHC-P IHC-F IF  100ul
上调基因4抗体  Anti-URG4   WB IHC-P IHC-F IF  100ul
尿皮质素UCN3抗体  Anti-Urocortin 3  WB IHC-P IHC-F IF  100ul
泛素融合降解样蛋白1抗体  Anti-UFD1L  WB IHC-P IHC-F IF  100ul
细胞生长调节蛋白CGREF1抗体  Anti-CGREF1  WB IHC-P IHC-F IF  100ul
富含亮酸软骨蛋白抗体  Anti-CHAD  WB IHC-P IHC-F IF  100ul
软骨蛋白样蛋白抗体  Anti-CHADL  WB IHC-P IHC-F IF  100ul
卷曲螺旋结构域蛋白CHCHD3抗体  Anti-CHCHD3  WB IHC-P IHC-F IF  100ul
卷曲螺旋结构域蛋白CHCHD10抗体  Anti-CHCHD10  WB IHC-P IHC-F IF  100ul
三腺苷受体P2X5抗体  Anti-P2RX5  WB IHC-P IHC-F IF  100ul
三腺苷受体P2X5b抗体  Anti-P2X5b  WB IHC-P IHC-F IF  100ul
三腺苷受体P2X6抗体  Anti-P2RX6  WB IHC-P IHC-F IF  100ul
突触富含脯酸膜蛋白7抗体  Anti-PRR7  WB IHC-P IHC-F IF  100ul
过氧化物酶体生成蛋白5相关蛋白抗体  Anti-PEX5L  WB IHC-P IHC-F IF  100ul
大鼠肾上腺嗜铬细胞瘤细胞;PC-12 [PC12]
婆罗门牛皮肤成纤维样细胞;BOS-4
CCL17 Others Human CCL17 / TARC / SCYA17 杆状病毒-昆虫细胞裂解液 (阳性对照)
HeLa[Chang Liver]细胞,张氏肝细胞 人结肠癌细胞,HRT-S1细胞 CM-R069大鼠肾成纤维细胞完全培养基100mL
人肝星形细胞HHSteC
CD80 Others Human B7-1 / CD80 人细胞裂解液 (阳性对照)
人气管上皮细胞cDNAHTEpiC cDNA
SLAMF1 Others Rat 大鼠 CD150 / SLAM / SLAMF1 人细胞裂解液 (阳性对照)
tTA基因修饰的小鼠畸胎瘤细胞(B);F9-CAG-tTA-3A1
QGY-7703细胞,人肝癌细胞 人羊膜细胞,WISH细胞 大鼠骨肉瘤细胞;UMR-106
KLE(人子宫内膜癌细胞) 5×106cells/瓶×2
Promocell C-28010 Mesenchymal Stem CellGrowth Medium, 间充质干细胞生长培养基(即用型) 500ml
磷酸化精子尾部结构蛋白抗体图片大鼠肾上腺嗜铬细胞瘤细胞;PC-12 [PC12]
婆罗门牛皮肤成纤维样细胞;BOS-4
CCL17 Others Human CCL17 / TARC / SCYA17 杆状病毒-昆虫细胞裂解液 (阳性对照)
HeLa[Chang Liver]细胞,张氏肝细胞 人结肠癌细胞,HRT-S1细胞 CM-R069大鼠肾成纤维细胞完全培养基100mL
人肝星形细胞HHSteC
CD80 Others Human B7-1 / CD80 人细胞裂解液 (阳性对照)
 

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