VECTASHIELD® with DAPI 防荧光萃灭封片剂(含DAPI)价格

供应商：欣博盛

库存：大量现货

英文名：VECTASHIELD® Antifade Mounting Mediumwith DAPI

保质期：请参考产品说明

保存条件：4°C

规格：10 ml

欣博盛独家代理品牌vectorlabs，公司成立时，那时市面上还没有单克隆抗体、生物素-亲和素产品以及凝集素产品。1976, Vectorlabs率先纯化出凝集素产品，并迅速成为世界上该类产品的主要供应商。次年，Vetorlabs推出了生物素-亲和素系统。由于生物素和亲和素分子间极强的相互作用力，因此可以在生物素化的分子和结合有标记分子的亲和素间形成近似共价键的连接。Vectorlabs可提供抗原修复试剂、封闭试剂、复染试剂、亲和素/链霉亲和素-酶结合物、ABC免疫组化试剂盒、免疫组化显色底物、免疫组化抗体等。

VECTASHIELD^® Antifade Mounting Media ——卓越的防淬灭封片剂产品

选择有效的封片剂对免疫荧光成像观察至关重要。荧光基团对荧光成像中的激发光和保存期间的光漂白和光淬灭十分敏感。使用正确的封片剂可以对样品实现短期或长期的保存。

VECTASHIELD^®Antifade防淬灭封片剂可以对样本提供卓越的防褪色和光漂白保护。VECTASHIELD^®和VECTASHIELD^®HardSet™Antifade封片剂是目前市场上产品性能优越、品质完善、领先于其他同类产品的一款封片剂产品，可以优秀的保留免疫组化工作流程的中最后的图像采集和样本中的目的信号。

产品特征

●抑制大多数荧光基团、染料、荧光蛋白和其他干扰物质的光漂白；

●折射率适宜；

●即用型试剂，无需加热操作；

●对长时间存放载玻片，仍具有抑制光漂白的作用；

●使用方便

●两种产品：含有核或细胞骨架染色或不含核或细胞骨架染色；

●具有硬化或非硬化两种形式。

VECTASHIELD^®封片剂与荧光团的兼容性

VECTASHIELD^®封片剂是用于免疫荧光应用中使用最广泛的抗荧光淬灭封片剂。目前有超过60,000篇参考文献引用了VECTASHIELD^®封片剂这款产品，这些文献数据表明该封片剂可与超过130种荧光基团和荧光标记物具有兼容性，突显出VECTASHIELD^®封片剂在这个应用中的重要性。

下图显示了与VECTASHIELD^®防淬灭封片剂搭配使用的最常见的荧光基团：

下表中列出的荧光化合物是用于免疫荧光实验中经常选择的荧光化合物，也是与VECTASHIELD^®防淬灭封片剂成功搭配使用的荧光化合物。从传统到现在，这些化合物的范围，跨越广泛的光谱范围，并应用与一系列的应用中，这充分展示了VECTASHIELD™试剂的多功能性。

有关VECTASHIELD^®产品可与超过130种荧光基团和荧光标记搭配使用的完整列表，

请访问网站：www.vectorlabs.com/vslist

Fluorophore
acridine orange	coumarin	Fluoro-Jade^®	NeuroTrace^®	Quantum dot/Qdot
Alexa Fluor^®350	dihydroethidium	Lucifer yellow	Nile red	SYTOX^® Green
Alexa Fluor^®680	DRAQ5™	LysoTracker^®	Oil red O	TAMRA
Atto^® dyes	Evans blue	LysoTracker^®Red	Pacific Blue™	thioflavin s
BODIPY^®	fast blue	MitoTracker^® Red	PicoGreen^®	TOTO^®-3

VECTASHIELD^®封片剂形式和应用

下面插图为我们的抗淬灭封片剂产品建立了应用程序。 VECTASHIELD^®防淬灭封片剂广泛用于荧光和荧光共聚焦等免疫荧光应用中，来保护传统和当代荧光基团的固有特性。

原始VECTASHIELD^®形式的多功能性解决了使用多种平台和荧光标记的实验室科研工作者和核心设施的需求。此外，VECTASHIELD^®封片剂也被认为是新兴技术领域的领导产品，如超分辨率显微镜（SRM）。

在当前正在进行的SRM技术中，VECTASHIELD^®防淬灭封片剂的性能在随机光学重建显微镜（STORM）和结构化照明显微镜（SIM）中具有十分有利的优势。

VECTASHIELD^®封片剂

VECTASHIELD^®防淬灭封片剂是一种基于甘油的水性封片固定剂，在载玻片上保持粘性液体形式，不凝固。封片后，样本不会很快变干，可以在几周内对样本进行观察，并且无需密封。为了延长储存时间，可以使用指甲油对样本片进行永久密封。

VECTASHIELD^® HardSet™防淬灭封片剂

VECTASHIELD^® HardSet™防淬灭封片剂是一种水溶性封片固定剂，室温下20分钟，即可硬化。这种封片剂操作简单、方便，无需使用指甲油对盖玻片进行固定，并且便于与油镜一起使用。可与有或没有DAPI/TRITC-phalloidin复染兼容使用。

产品应用举例

组织切片		Rat muscle (FFPE): GFAP (red) and NF200 (green). Counterstained and coverslipped with VECTASHIELD^® Mounting Medium with DAPI (blue). The double IF was performed by Dr. Lynn Dong, Dept of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
超分辨率图像		Structured illumination super resolution photomicro-graph of a ciliated bovine airway epithelial cell labeled for acetylated alpha tubulin (cilia marker; green), and phosphodiesterase 5 (red). Coverslipped using VECTASHIELD^® HardSet™ Antifade Mounting Medium with DAPI (H-1200; nuclei, blue). Sample prepared and image taken by Michael E. Price, University of Nebraska Medical Center. With assistance of Janice A. Taylor and James R. Talaska, Advanced Microscopy Core Facility, University of Nebraska Medical Center, NE, USA.
细胞培养		Human embryonic stem cell (hESC)-derived neurons stained with Brn3a (Green), Peripherin (Red), Beta-3 Tubulin (Magenta). Coverslipped using VECTASHIELD^® Antifade Mounting Medium with DAPI (H-1200; nuclei, blue). Image courtesy of Michael Yee, Kinchington Lab, Department of Ophthalmology, University of Pittsburgh, USA

产品订购信息

Product

No Counterstain

With DAPI Counterstain

With PI Counterstain

TRITC-Phalloidin

VECTASHIELD^®Mounting Medium

(non-hardening)

H-1000

H-1200

H-1300

VECTASHIELD^®HardSet™ Mounting Medium (hardening)

H-1400

H-1500

H-1600

文献引用

1.Sanders L H, Laganière J, Cooper O, et al. LRRK2 mutations cause mitochondrial DNA damage in iPSC-derived neural cells from Parkinson's disease patients: reversal by gene correction[J]. Neurobiology of disease, 2014, 62: 381-386.
2.Mahadevaiah S K, Costa Y, Turner J M A. Using RNA FISH to study gene expression during mammalian meiosis[M]//Meiosis. Humana Press, Totowa, NJ, 2009: 433-444.
3.Schnell M A, Zhang Y, Tazelaar J, et al. Activation of innate immunity in nonhuman primates following intraportal administration of adenoviral vectors[J]. Molecular Therapy, 2001, 3(5): 708-722.
4.Ippolito D M, Eroglu C. Quantifying synapses: an immunocytochemistry-based assay to quantify synapse number[J]. JoVE (Journal of Visualized Experiments), 2010 (45): e2270.
5.Guerreiro A, Meraldi P. AA344 and AA345 antibodies recognize the microtubule network in human cells by immunofluorescence[J]. Antibody Reports, 2019, 2(1): e17-e17.
6.Englund D A, Peck B D, Murach K A, et al. Resident muscle stem cells are not required for testosterone-induced skeletal muscle hypertrophy[J]. American Journal of Physiology-Cell Physiology, 2019, 317(4): C719-C724.
7.Englund D A, Peck B D, Murach K A, et al. Resident muscle stem cells are not required for testosterone-induced skeletal muscle hypertrophy[J]. American Journal of Physiology-Cell Physiology, 2019, 317(4): C719-C724.
8.Fagot D, Pham D M, Laboureau J, et al. Crocin, a natural molecule with potentially beneficial effects against skin ageing[J]. International journal of cosmetic science, 2018, 40(4): 388-400.
9.Sandell L, Inman K, Trainor P. DAPI Staining of Whole-Mount Mouse Embryos or Fetal Organs[J]. Cold Spring Harbor Protocols, 2018, 2018(10): pdb. prot094029.
10.Kim D, Kwon S. Mechanical load increase–induced changes in cytoskeletal structure and cellular barrier function in human cerebral endothelial cells[J]. Biotechnology and bioengineering, 2018, 115(10): 2624-2631.

该产品被引用文献

1.Sanders L H, Laganière J, Cooper O, et al. LRRK2 mutations cause mitochondrial DNA damage in iPSC-derived neural cells from Parkinson's disease patients: reversal by gene correction[J]. Neurobiology of disease, 2014, 62: 381-386.
2.Mahadevaiah S K, Costa Y, Turner J M A. Using RNA FISH to study gene expression during mammalian meiosis[M]//Meiosis. Humana Press, Totowa, NJ, 2009: 433-444.
3.Schnell M A, Zhang Y, Tazelaar J, et al. Activation of innate immunity in nonhuman primates following intraportal administration of adenoviral vectors[J]. Molecular Therapy, 2001, 3(5): 708-722.
4.Ippolito D M, Eroglu C. Quantifying synapses: an immunocytochemistry-based assay to quantify synapse number[J]. JoVE (Journal of Visualized Experiments), 2010 (45): e2270.
5.Guerreiro A, Meraldi P. AA344 and AA345 antibodies recognize the microtubule network in human cells by immunofluorescence[J]. Antibody Reports, 2019, 2(1): e17-e17.
6.Englund D A, Peck B D, Murach K A, et al. Resident muscle stem cells are not required for testosterone-induced skeletal muscle hypertrophy[J]. American Journal of Physiology-Cell Physiology, 2019, 317(4): C719-C724.
7.Englund D A, Peck B D, Murach K A, et al. Resident muscle stem cells are not required for testosterone-induced skeletal muscle hypertrophy[J]. American Journal of Physiology-Cell Physiology, 2019, 317(4): C719-C724.
8.Fagot D, Pham D M, Laboureau J, et al. Crocin, a natural molecule with potentially beneficial effects against skin ageing[J]. International journal of cosmetic science, 2018, 40(4): 388-400.
9.Sandell L, Inman K, Trainor P. DAPI Staining of Whole-Mount Mouse Embryos or Fetal Organs[J]. Cold Spring Harbor Protocols, 2018, 2018(10): pdb. prot094029.
10.Kim D, Kwon S. Mechanical load increase–induced changes in cytoskeletal structure and cellular barrier function in human cerebral endothelial cells[J]. Biotechnology and bioengineering, 2018, 115(10): 2624-2631.

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