人ES/iPS细胞监测试剂盒

保存条件 ：2-10℃

保质期 ：详见说明书

英文名 ：Human ES/iPS Cell Monitoring Kit

库存 ：999

供应商 ：北京拜尔迪生物技术有限公司

规格 ：96tests

This product is for research use only. Do not administer it to human.rBC2LCN lectin exhibits significant affinity to a mucin-type O-glycan sugar chain called H-type3 (Fuc α1-2Gal β1-3GalNAc) displayed on the podocalyxin binds to the surface of human pluripotent stem cells (hPSCs), human ES cells and human iPS cells. Therefore rBC2LCN is reported as a marker of hPSCs.The glycoprotein recognized by rBC2LCN is released from hPSCs into cell culture supernatant. This kit can quantitatively determine the released glycoprotein by rBC2LCN-antibody sandwich assay and estimate the number of hPSCs. Because, cell culture supernatants, but not cells itself, are used as sample targeted for assay, it is easily capable of monitoring the increase and decrease of hPSCs in a noninvasive manner during continuous cell culture.· Increase or decrease in the number of undifferentiated cells can be monitored by analyzing culture the supernatants of undifferentiated cells that are undergoing differentiation.· Culture supernatants (50μL) are used for the assay. Cells are not processed for the analysis and can remain in culture.· As an ELISA-based method is used, multiple samples can be processed using a simple procedure