样本：血清/血浆/组织匀浆液

库存：1000

适应物种：Rabbit

应用：科研使用

检测方法：ELISA

检测限：31.25 - 2000 pg/ml

供应商：康朗生物

规格：96T

Rabbit CCL2 / MCP1 ELISA Kit (Sandwich ELISA) - LS-F37062

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LS-F37062-1
1 plate
$865 USD
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Product Description

LS-F37062 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Rabbit CCL2 / MCP1 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of CCL2 / MCP1 as low as 18.75 picograms per milliliter.
About CCL2 / MCP1
CCL2 / MCP1 is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. Uniprot: P13500 NCBI: NM_002982 NP_002973.1

Synonyms: CCL2 ElisaKit, C-C motif chemokine 2 ElisaKit, Chemokine (C-C motif) ligand 2 ElisaKit, GDCF-2 ElisaKit, HC11 ElisaKit, HSMCR30 ElisaKit, MCP1 ElisaKit, SCYA2 ElisaKit, Small-inducible cytokine A2 ElisaKit, Monocyte secretory protein JE ElisaKit, MCAF ElisaKit, MCP-1 ElisaKit, Monocyte chemotactic protein 1 ElisaKit, SMC-CF ElisaKit

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Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

CCL2 / MCP1
Reactivity

Rabbit
Manual

Intended Sample Types

Plasma, Serum, Tissue Homogenates
Format

96-Well Strip Plate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

31.25 - 2000 pg/ml
Sensitivity

18.75
Precision

Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage

Short term: 4°C; Long term: see manual.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

Adhesive Plate Sealers
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP-Streptavidin Conjugate Diluent
Sample Diluent
TMB Substrate
Stop Solution
Wash Buffer (25x)
Standard (Lyophilized)

Background

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- MCP-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MCP-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-1 can be calculated.
Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
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