产品详情
文献和实验
相关推荐
CAS号 ：Optiprep
保质期 ：Optiprep
保存条件 ：1893
供应商 ：1893
库存 ：大量
英文名 ：Recombinant Mouse Monocyte Chemotactic Protein-1 (CCL2)
规格 ：250ml
OptiPrep密度梯度分离液OptiPrep是一种60%碘克沙醇在水中的无菌内毒素测试溶液，密度为1.320 g/ml。碘克沙醇是一种X射线造影剂，因此经过严格的临床测试。它是非离子型，对细胞无毒，代谢惰性。碘克沙醇溶液可在所有有用密度下均为等渗溶液，该溶液具有低粘度和渗透压。
OptiPrep的高密度有助于通过连续或不连续梯度或通过简单的密度屏障从致密负载区浮选分离电池。
Optiprep分离液是一种无菌的、内毒素含量很低的多用途分离液，可用于各种细胞、细胞器、病毒、生物大分子等的离心分离。
特点：
OptiprepTM 分离液是Axis-shield公司最xin开发出的全能新型分离液，OptiprepTM 分离液的主要成分是碘克沙醇（odixanol），是一种高亲水性非离子型的物质，这种主要成分决定了OptiprepTM 分离液是一种高密度、低粘度的溶液。而且，它的密度和渗透性成线形关系，在加入适当浓度的缓冲液或基本培养基，如蔗糖、Hepes或PRMI后，OptiprepTM 能形成连续的或不连续的等渗的密度梯度溶液，可将各种细胞、细胞器、脂蛋白分离开。经科研和临床实验证明，OptiprepTM 和常规的分离液Ficoll、Percoll、蔗糖及氯化铯（CsCl）相比，OptiprepTM 分离液不仅分离纯化效果好，而且对各种细胞、细胞器的生命活性无任何影响。
技术参数：
成分：Iodixanol：60%（w/v）
密度（Density）：1.320±0.001g/ml
渗透压(Osmolality):170±15mOsm
内毒素含量（Endotoxins）：<1.0EU/ml
51种可用于细胞分离的协议。提高细胞器的分辨率。低粘度、等渗梯度可快速有效地分离预成型梯度中的主要细胞器。
- C01 – Preparation of density gradient solutions
- C02 – Preparation of gradients for cells
- C03 – Mononuclear cells, lymphocytes, monocytes and polymorphonuclear leukocytes from blood: a methodological review
- C04 – Isolation of mononuclear cells from human blood by sedimentation on to a density barrier
- C05 – Isolation of human peripheral blood mononuclear cells by flotation (iodixanol mixer technique)
- C06 – Isolation of human peripheral blood mononuclear cells by flotation (low density iodixanol density barrier)
- C07 – Isolation of rat blood mononuclear cells by flotation (iodixanol mixer technique)
- C08 – Isolation of mononuclear cells from mouse blood by flotation (iodixanol mixer technique)
- C09 – Isolation of ruminant and equine peripheral blood mononuclear cells in iodixanol gradients
- C10 – Separation of monocytes from a leukocyte-rich plasma by flotation through a discontinuous iodixanol gradient
- C11 – Isolation of a monocyte-rich fraction from whole human blood by iodixanol barrier flotation
- C12 – Isolation of human polymorphonuclear leukocytes (granulocytes) from a leukocyte rich plasma in a discontinuous iodixanol gradient
- C13 – Isolation (removal) of human platelets (thrombocytes) on an iodixanol barrier
- C14 – Removal of non-viable cells from a cell suspension
- C15 – Fractionation of a mixed population of cells
- C16 – Purification of Islets of Langerhans from porcine, primate and rodent pancreas in a discontinuous iodixanol gradient
- C17 – Purification and processing of animal (non-human) and avian spermatozoa
- C18 – Purification of viable human spermatozoa in iodixanol or Nycodenz® gradients
- C19 – Purification of intact plant protoplasts by density gradient flotation
- C20 – Purification of leukocyte fractions from rodent/rabbit peritoneal exudates
- C21 – Dendritic cells from tissues by flotation through a low-density barrier
- C22 – Isolation of dendritic cells from tissues by a mixer technique
- C23 – Isolation of a mouse motoneuron enriched fraction from spinal cord on a density barrier
- C24 – Isolation of a progenitor cell-enriched fraction
- C25 – Hepatic non-parenchymal (stellate, Kupffer and endothelial) cells – a short methodological survey
- C26 – Purification of hepatic non-parenchymal cells on a density barrier
- C27 – Preparation of stellate cells from liver and pancreas
- C28 – Enrichment of hepatic Kupffer cells in a discontinuous gradient
- C29 – Isolation of cells from pulmonary tissue Endothelial from epithelial cells, type I epithelial cells and macrophages
- C30 – Isolation of rat alveolar type II pneumocytes, lymphoid and myeloid cells
- C31 – Purification of protozoa
- C32 – Purification of malarial parasites (Plasmodium falciparum, Plasmodium berghei, Plasmodium vivax, and Plasmodium yoelii)
- C33 – Purification of Toxoplasma gondii from cell cultures (Part A) Separation of sporocysts and oocyst walls (Part B)
- C34 – Fractionation and analysis of normal erythrocytes, sickle cells and reticulocytes; removal of erythrocytes from blood and bone marrow
- C35 – Isolation of neuroglia, inflammatory and glial cells from neural tissue
- C36 – Isolation of brain motoneurons
- C37 – Isolation of renal cells: (1) Proximal tubule cells and (2) Interstitial cells and thin loop of Henlé cells
- C38 – (I) Maintenance of cells in suspension for chemical/physical measurements (II) Microfluidic cell encapsulation/cell sorting
- C39 – Purification of bacteria from soil, stream-water, clinical specimens and food
- C40 – Isolation of mononuclear cells from tissues
- C41 – Isolation of dendritic cells from tissues by sedimentation on to a density barrier
- C42 – Purification of macrophages and foam cells
- C43 – Isolation of mononuclear cells from rat, mouse and rabbit blood on a density barrier
- C44 – Isolation of polymorphonuclear leukocytes (granulocytes) from rat, mouse, guinea pig and rabbit blood
- C45 – Isolation of peripheral blood mononuclear cells from non-human primates
- C46 – Isolation of monocytes from a human leukocyte-rich plasma on a density barrier
- C47 – Purification of enterochromaffin-like (ECL) cells from gastric mucosa
- C48 – Fractionation of parietal and chief cells from the gastric mucosa
- C49 – Purification of bacteria and bacterial minicells from cultured cells
- C50 – Fractionation of coelomocyte cell populations from sea urchins
- C51 – Analysis of gradients
- Cell-index-Serumwerk
62种方案可用于亚细胞器和膜的分离。
OptiPrep是病毒纯化的理想解决方案。
使用OptiPrep梯度纯化的病毒具有传染性：颗粒数比至少是CsCl梯度的100倍。
38种可用于病毒净化的协议
13个大分子和脂蛋白纯化协议。使用特定的LAL试验检查每批OptiPrep的内毒素水平。我们的目标是生产内毒素水平低于或等于0.13 IU/ml的批次。
对于生产的每批产品，在“诊断”中提供显示密度和内毒素水平实际值的分析证书。塞伦沃克。根据欧洲药典，我们也要求无菌。
OptiPrep的生产、包装和发布符合GMP和ISO13485。
应用
隔离：
哺乳动物和非哺乳动物细胞
亚细胞器
质膜和结构域
膜泡与胞浆
非哺乳动物来源的细胞器
病毒
血浆脂蛋白
蛋白质和蛋白质复合物
质粒DNA
核糖核蛋白
分析：
膜转运与细胞凋亡
信号
出胞
OptiPrep is a ready-made, sterile and endotoxin tested
solution with the following specifications:
solution with the following specifications:
| Iodixanol | 60% (w/v) |
| Density | 1.320 ± 0.001 g/ml |
| Endotoxins | < 1.0 IU/ml |
OptiPrep is supplied as
a sterile solution in the following package sizes:
a sterile solution in the following package sizes:
| Prod. No. 1893 | 1 x 250 ml |
上海研卉生物科技有限公司
实名认证
金牌会员
入驻年限：10年
