c-fos抗体

克隆性 ：多克隆

适应物种 ：H, R

应用范围 ：WB, ICC

抗体英文名 ：Anti-c-Fos Antibody

规格 ：100ug

c-fos抗体CBL440，Anti-c-Fos Antibody
Anti-c-Fos Antibody is a Sheep Polyclonal Antibody for detection of c-Fos also known as G0/G1 Switch Regulatory Protein 7 & has been validated in WB, ICC.

重要规格表

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, R	WB, ICC	Sh	Purified	Polyclonal Antibody

Description
Catalogue Number	CBL440
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-c-Fos Antibody
Alternate Names	G0/G1 Switch Regulatory Protein 7

Product Information
Format	Purified
Presentation	The purified immunoglobulin is presented at a concentration of 100μg/ml in phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Applications
Application	Anti-c-Fos Antibody is a Sheep Polyclonal Antibody for detection of c-Fos also known as G0/G1 Switch Regulatory Protein 7 & has been validated in WB, ICC.
Key Applications	Western Blotting Immunocytochemistry
Application Notes	Immunoblotting: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein. CBL 440 detects a band at 55-60 kDa on immunoblots of stimulated HeLa and HL-60 lysates. Immunocytochemistry: CBL 440 has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (where CBL 440 detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala-Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.
Host	Sheep
Specificity	c-fos is a 55 kDa nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents. c-fos is also induced in neuronal tissue following drug induced seizures. KNOWN SPECIES CROSS REACTIVITY: Known to cross-react with rat
Species Reactivity	Human Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_005252.2
Entrez Gene Summary	The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Gene Symbol	FOS G0S7 c-fos
UniProt Number	P01100
UniProt Summary	FUNCTION: SwissProt: P01100 # Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SIZE: 380 amino acids; 40695 Da SUBUNIT: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB (By similarity). SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: P01100 ## Belongs to the bZIP family. Fos subfamily. & Contains 1 bZIP domain.

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.