重组tau蛋白突变型， Tau441价格_品牌:ProSpec-丁香通官网

供应商：上海研卉生物科技有限公司

库存：大量

规格：100ug

重组tau蛋白突变型， Tau441

Tau Protein

Active Human Recombinant Tau441 (2N4R), P301S mutant Protein Monomer

Catalog No. SPR-327

Expression System	E. coli
Tags	No tag

Product Name	Tau Protein
Description	Active Human Recombinant Tau441 (2N4R), P301S mutant Protein Monomer
Applications	WB, SDS-PAGE, In vivo assay, In vitro assay
Concentration	Lot/batch specific. See included datasheet.
Conjugates	No tag
Nature	Recombinant
Species	Human
Expression System	E. coli
Amino Acid Sequence	MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKESPLQT PTEDGSEEPG SETSDAKSTP TAEDVTAPLV DEGAPGKQAA AQPHTEIPEG TTAEEAGIGD TPSLEDEAAG HVTQARMVSK SKDGTGSDDK KAKGADGKTK IATPRGAAPP GQKGQANATR IPAKTPPAPK TPPSSGEPPK SGDRSGYSSP GSPGTPGSRS RTPSLPTPPT REPKKVAVVR TPPKSPSSAK SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVSGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE NAKAKTDHGA EIVYKSPVVS GDTSPRHLSN VSSTGSIDMV DSPQLATLAD EVSASLAKQG L
Protein Length	Full Length
Biological Activity	Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers.
Field of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Storage Buffer	10 mM HEPES, 100 mM NaCl pH 7.4
Storage Temperature	-80ºC
Shipping Temperature	Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Purification	Ion-exchange Purified
Specificity	~45.8 kDa
Cite This Product	Human Recombinant Tau Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-327)

Alternative Names	Active tau monomer, active tau protein monomer, active tau protein, microtubule-associated protein tau, MAPT, MAP, microtubule-associated protein, tau-441, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 2, tubulin-associated unit
Research Areas	Alzheimer's Disease, Axon Markers, Cell Markers, Cell Signaling, Cytoskeleton, Microtubules, MT Associated Proteins, Neurodegeneration, Neuron Markers, Neuroscience, Tangles & Tau
Cellular Localization	Cytoplasm, Axolemma, Axolemma Plasma Membrane, Axon, Cell Body, Cell membrane, Cytoplasmic Ribonucleoprotein Granule, Cytoplasmic Side, Cytoskeleton, Cytosol, Dendrite, Growth cone, Microtubule, Microtubule Associated Complex, Neurofibrillary Tangle, Neuronal Cell Body, Nuclear Periphery, Nuclear Speck, Nucleus, Peripheral membrane protein, Plasma Membrane, Tubulin Complex
Accession Number	NP_005901.2
Gene ID	4137
Swiss Prot	P10636
Scientific Background	Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). 2N4R, or Tau-441 is the full length tau protein. P301S is a mutation encoded by exon 10 (4) that impairs the ability of tau to assemble microtubules (5).
References	1. www.alz.org/alzheimers-dementia/facts-figures 2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907) 3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497. 4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18. 5. Bugiani, O. et al. (1999). J Neuropathol Exp Neurol. 58(6):667-77.

SDS-PAGE of ~67 kDa Active Human Tau Protein 2N4R P301S Monomer (SPR-327). Lane 1: MW ladder. Lane 2: Active Tau Protein Monomer (SPR-327)

Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in active tau monomers (SPR-327). A greater increase in fluorescence is seen when 50 uM active monomer (SPR-327) is combined with 10 nM of active fibrils (SPR-329 or SPR-330), as the active fibrils seed the formation of new fibrils from the pool of active monomers. Thioflavin T ex = 450 nm, em = 485 nm.