pCambia1305.2

库存 ：大量

英文名 ：pCambia1305.2

保质期 ：6个月

供应商 ：上海钰博生物科技有限公司

保存条件 ：-20℃

规格 ：1ug/5ug

产品编号	载体名称
YB0210	pCambia1305.2

pCambia 1305.2载体基本信息

载体名称:	pCambia1305.2, pCambia 1305.2
质粒类型:	植物表达载体
高拷贝/低拷贝:	低拷贝
启动子:	CAMV35S
克隆方法:	多克隆位点，限制性内切酶
载体大小:	11922 bp
5' 测序引物及序列:	35S promoter: CTATCCTTCGCAAGACCCTTC
3' 测序引物及序列:	--
载体标签:	带有分泌信号肽的Gus序列。
载体抗性:	卡那
筛选标记:	HPTII
备注:	--
产品目录号:	1305.2
稳定性:	稳定表达
组成型:	非组成型
病毒/非病毒:	非病毒

pCambia 1305.2载体质粒图谱和多克隆位点信息

 

pCambia 1305.2载体序列

 

LOCUS       pCAMBIA1305.2          11922 bp ds-DNA    circular SYN  DEFINITION  Agrobacterium binary vector for plant transformation, with             hygromycin- and kanamycin-resistance and secreted GUSPlus(TM) genes.ACCESSION   AF354046VERSION     .KEYWORDS    pCAMBIA1305.2SOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructCOMMENT     This file is created by Vector NTICOMMENT     The GenBank record was corrected by inserting a G at position 4815.FEATURES             Location/Qualifiers     source          1..11922                     /organism="synthetic DNA construct"                     /lab_host="Plant Cells"                     /mol_type="other DNA"     CDS             2..82                     /codon_start=1                     /product="signal sequence from?Oryza sativa glycine-rich                      cell wall protein"                     /label="GRP signal sequence"                     /translation="MATTKHLALAILVLLSIGMTTSARTLL"     CDS             join(83..91,282..2099)                     /codon_start=1                     /gene="                     "                     /product="beta-glucuronidase"                     /label="GUSPlus(TM)"                     /label="codon-optimized Staphylococcus gusA?gene?with a                      catalase intron to ensure expression in plants but not                      bacteria"                     /protein_id="                     "     intron          92..281                     /label="cat1 intron"                     /label="castor bean catalase intron, modified"     CDS             2106..2123                     /codon_start=1                     /product="6xHis affinity tag"                     /label="6xHis"                     /translation="HHHHHH"     terminator      2155..2407                     /label="NOS terminator"                     /label="nopaline synthase terminator and poly(A) signal"     misc_feature    2429..2453                     /label="RB T-DNA repeat"                     /label="right border repeat from nopaline C58 T-DNA"     CDS             3753..4382                     /codon_start=1                     /product="stability protein from Pseudomonas plasmid pVS1"                     /label="pVS1 StaA"     CDS             4811..5884                     /codon_start=1                     /product="replication protein from Pseudomonas plasmid                      pVS1"                     /label="pVS1 RepA"                     /label="                     "                     /protein_id="                     "     misc_feature    6488..6628                     /label="bom"                     /label="basis of mobility region from pBR322"     rep_origin      complement(6814..7402)                     /direction=LEFT                     /label="ori"                     /label="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(7489..8283)                     /codon_start=1                     /gene="aphA-3"                     /product="aminoglycoside phosphotransferase"                     /label="KanR"                     /label="confers resistance to kanamycin"                     /protein_id="                     "     misc_feature    8708..8732                     /label="LB T-DNA repeat"                     /label="left border repeat from nopaline C58 T-DNA"     polyA_signal    8810..8984                     /label="CaMV poly(A) signal"                     /label="cauliflower mosaic virus polyadenylation signal"     CDS             complement(9024..10049)                     /codon_start=1                     /product="hygromycin B phosphotransferase"                     /label="HygR"                     /label="confers resistance to hygromycin"     promoter        complement(10117..10794)                     /label="CaMV 35S promoter (enhanced)"                     /label="cauliflower mosaic virus 35S promoter with a                      duplicated enhancer region"     promoter        11021..11051                     /label="lac promoter"                     /label="promoter for the E. coli lac operon"     protein_bind    11059..11075                     /bound_moiety="lac repressor encoded by lacI"                     /label="lac operator"                     /label="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."     primer_bind     11083..11099                     /label="M13 rev"                     /label="common sequencing primer, one of multiple similar                      variants"     CDS             11095..11328                     /codon_start=1                     /gene="lacZ (truncated)"                     /product="LacZ-alpha fragment of?beta-galactosidase"                     /label="lacZ-alpha"                     /protein_id="                     "                     /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"     misc_feature    11109..11165                     /gene="                     "                     /label="MCS"                     /label="pUC18/19 multiple cloning site"     primer_bind     complement(11169..11185)                     /label="M13 fwd"                     /label="common sequencing primer, one of multiple similar                      variants"     promoter        11562..11907                     /label="CaMV 35S promoter"                     /label="strong constitutive promoter from cauliflower mosaic                     virus"ORIGIN        1 catggctact actaagcatt tggctcttgc catccttgtc ctccttagca ttggtatgac       61 caccagtgca agaaccctcc tagatctgag ggtaaatttc tagtttttct ccttcatttt      121 cttggttagg acccttttct ctttttattt ttttgagctt tgatctttct ttaaactgat      181 ctatttttta attgattggt tatggtgtaa atattacata gctttaactg ataatctgat      241 tactttattt cgtgtgtcta tgatgatgat gatagttaca gaaccgacga actagtctgt      301 acccgatcaa caccgagacc cgtggcgtct tcgacctcaa tggcgtctgg aacttcaagc      361 tggactacgg gaaaggactg gaagagaagt ggtacgaaag caagctgacc gacactatta      421 gtatggccgt cccaagcagt tacaatgaca ttggcgtgac caaggaaatc cgcaaccata      481 tcggatatgt ctggtacgaa cgtgagttca cggtgccggc ctatctgaag gatcagcgta      541 tcgtgctccg cttcggctct gcaactcaca aagcaattgt ctatgtcaat ggtgagctgg      601 tcgtggagca caagggcgga ttcctgccat tcgaagcgga aatcaacaac tcgctgcgtg      661 atggcatgaa tcgcgtcacc gtcgccgtgg acaacatcct cgacgatagc accctcccgg      721 tggggctgta cagcgagcgc cacgaagagg gcctcggaaa agtcattcgt aacaagccga      781 acttcgactt cttcaactat gcaggcctgc accgtccggt gaaaatctac acgaccccgt      841 ttacgtacgt cgaggacatc tcggttgtga ccgacttcaa tggcccaacc gggactgtga      901 cctatacggt ggactttcaa ggcaaagccg agaccgtgaa agtgtcggtc gtggatgagg      961 aaggcaaagt ggtcgcaagc accgagggcc tgagcggtaa cgtggagatt ccgaatgtca     1021 tcctctggga accactgaac acgtatctct accagatcaa agtggaactg gtgaacgacg     1081 gactgaccat cgatgtctat gaagagccgt tcggcgtgcg gaccgtggaa gtcaacgacg     1141 gcaagttcct catcaacaac aaaccgttct acttcaaggg ctttggcaaa catgaggaca     1201 ctcctatcaa cggccgtggc tttaacgaag cgagcaatgt gatggatttc aatatcctca     1261 aatggatcgg cgccaacagc ttccggaccg cacactatcc gtactctgaa gagttgatgc     1321 gtcttgcgga tcgcgagggt ctggtcgtga tcgacgagac tccggcagtt ggcgtgcacc     1381 tcaacttcat ggccaccacg ggactcggcg aaggcagcga gcgcgtcagt acctgggaga     1441 agattcggac gtttgagcac catcaagacg ttctccgtga actggtgtct cgtgacaaga     1501 accatccaag cgtcgtgatg tggagcatcg ccaacgaggc ggcgactgag gaagagggcg     1561 cgtacgagta cttcaagccg ttggtggagc tgaccaagga actcgaccca cagaagcgtc     1621 cggtcacgat cgtgctgttt gtgatggcta ccccggagac ggacaaagtc gccgaactga     1681 ttgacgtcat cgcgctcaat cgctataacg gatggtactt cgatggcggt gatctcgaag     1741 cggccaaagt ccatctccgc caggaatttc acgcgtggaa caagcgttgc ccaggaaagc     1801 cgatcatgat cactgagtac ggcgcagaca ccgttgcggg ctttcacgac attgatccag     1861 tgatgttcac cgaggaatat caagtcgagt actaccaggc gaaccacgtc gtgttcgatg     1921 agtttgagaa cttcgtgggt gagcaagcgt ggaacttcgc ggacttcgcg acctctcagg     1981 gcgtgatgcg cgtccaagga aacaagaagg gcgtgttcac tcgtgaccgc aagccgaagc     2041 tcgccgcgca cgtctttcgc gagcgctgga ccaacattcc agatttcggc tacaagaacg     2101 ctagccatca ccatcaccat cacgtgtgaa ttggtgacca gctcgaattt ccccgatcgt     2161 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt     2221 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg     2281 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata     2341 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta     2401 ctagatcggg aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag     2461 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg     2521 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca     2581 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa     2641 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg     2701 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca     2761 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga     2821 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt     2881 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca     2941 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg     3001 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc     3061 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg     3121 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc     3181 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca     3241 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg     3301 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga     3361 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc     3421 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa     3481 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag     3541 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc     3601 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa     3661 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga     3721 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga     3781 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg     3841 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg     3901 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg     3961 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact     4021 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt     4081 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg     4141 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg     4201 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc     4261 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg     4321 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt     4381 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc     4441 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa     4501 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg     4561 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat     4621 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc     4681 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc     4741 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg     4801 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg     4861 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc     4921 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg     4981 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc     5041 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga     5101 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga     5161 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga     5221 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga     5281 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt     5341 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt     5401 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac     5461 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg     5521 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa     5581 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg     5641 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa     5701 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg     5761 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc     5821 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc     5881 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg     5941 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa     6001 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga     6061 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct     6121 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa     6181 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc     6241 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca     6301 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt     6361 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc     6421 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt     6481 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta     6541 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag     6601 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct     6661 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     6721 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     6781 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     6841 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     6901 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     6961 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     7021 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     7081 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     7141 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     7201 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     7261 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     7321 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     7381 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     7441 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc     7501 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa     7561 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc     7621 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt     7681 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc     7741 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc     7801 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc     7861 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa     7921 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata     7981 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg     8041 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc     8101 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata     8161 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc     8221 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc     8281 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat     8341 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca     8401 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg     8461 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc     8521 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg     8581 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg     8641 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc     8701 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca     8761 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt     8821 tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata     8881 caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc     8941 taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc     9001 gacagatccg gtcggcatct actctatttc tttgccctcg gacgagtgct ggggcgtcgg     9061 tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg     9121 gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc     9181 tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga     9241 ccaatgcgga gcatatacgc ccggagtcgt ggcgatcctg caagctccgg atgcctccgc     9301 tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg     9361 gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg     9421 cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact     9481 tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg     9541 acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg     9601 aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc     9661 gtctggctaa gatcggccgc agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca     9721 gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg     9781 caataggtca ggctctcgct aaactcccca atgtcaagca cttccggaat cgggagcgcg     9841 gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt     9901 acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc     9961 tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca    10021 gacgtcgcgg tgagttcagg ctttttcata tctcattgcc ccccgggatc tgcgaaagct    10081 cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa    10141 tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc gtcatccctt    10201 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct    10261 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca    10321 tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc accttccttt    10381 tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag gaggtttccc    10441 gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac tgtatctttg    10501 atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca atccacttgc    10561 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca    10621 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga    10681 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct    10741 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc    10801 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc    10861 accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg cgttggccga    10921 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg    10981 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg    11041 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc    11101 atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg caggcatgca    11161 agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa    11221 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc    11281 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc    11341 ttggatcaga ttgtcgtttc ccgccttcag tttagcttca tggagtcaaa gattcaaata    11401 gaggacctaa cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga    11461 ctcaatgaca agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc    11521 aaaaatatca aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg    11581 gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag    11641 atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc    11701 gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc    11761 gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc    11821 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa    11881 ggaagttcat ttcatttgga gagaacacgg gggactcttg ac//