Human BMP12 / GDF7 ELISA Kit (Sandwich ELISA) - LS-F35346

价格： $990

品牌：LSBio

货号：LS-F35346

产品详情

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Mouse BMP12 / GDF7 ELISA Kit (Sandwich ELISA) - LS-F35347
Type: Quantitative Sandwich ELISA
Format: 96-Well Strip Plate
Reactivity: Mouse
Range: 15.625-1000 pg/ml
Detection: Colorimetric - 450nm (TMB)
Sample Types: Serum, Tissue Homogenates, Plasma

Product Description

LS-F35346 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Human BMP12 / GDF7 in samples of Plasma, Serum and Tissue Homogenates. It is based upon a Sandwich assay principle and can be used to detect levels of BMP12 / GDF7 as low as 37.5 picograms per milliliter.
About BMP12 / GDF7
BMP12 / GDF7 is a member of the bone morphogenetic protein (BMP) family. BMPs belong to the transforming growth factor-beta superfamily of secreted signalling molecules that regulate diverse processes in growth, repair and embryonic development. In mouse, this gene functions as an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord. Uniprot: Q7Z4P5 NCBI: NM_182828 NP_878248.2

Synonyms: GDF7 ElisaKit, BMP-12 ElisaKit, BMP12 ElisaKit, GDF-7 ElisaKit

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Specifications

Type

Sandwich ELISA (enzyme-linked immunosorbent assay) kit
Target

BMP12 / GDF7
Reactivity

Human
Manual

Intended Sample Types

Plasma, Serum, Tissue Homogenates
Format

96-Well Strip Plate
Detection

Colorimetric - 450nm (TMB)
Measurement

Quantitative
Detection Range

62.5 - 4000 pg/ml
Sensitivity

37.5
Precision

Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage

Short term: 4°C; Long term: see manual.
Quality Assurance

Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Kit Components

Adhesive Plate Sealers
Coated 96-well Strip Plate
Detection Antibody Diluent
Biotinylated Detection Antibody (100x)
HRP-Streptavidin Conjugate (100x)
HRP-Streptavidin Conjugate Diluent
Sample Diluent
TMB Substrate
Stop Solution
Wash Buffer (25x)
Standard (Lyophilized)

Background

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- GDF7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- GDF7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDF7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDF7 can be calculated.
Restrictions

For research use only.
Guarantee

This elisa kit carries the LSBio 100% Guarantee.
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