DNA合成检测，APC BrdU Flow Kit价格

英文名：APC BrdU Flow Kit

保存条件：低温

规格：50T

DNA合成检测，APC BrdU Flow Kit

Description

The immunofluorescent staining of incorporated bromodeoxyuridine (BrdU) and flow cytometric analysis provide a high-resolution technique to determine the frequency and nature of individual cells that have synthesized DNA. In this method, BrdU (an analog of the DNA precursor thymidine) is incorporated into newly synthesized DNA by cells entering and progressing through the S (DNA synthesis) phase of the cell cycle.The incorporated BrdU is stained with specific anti-BrdU fluorescent antibodies. The levels of cell-associated BrdU are then measured by flow cytometry. Often, staining with a dye that binds to total DNA such as 7-amino-actinomycin D (7-AAD) is coupled with immunofluorescent BrdU staining. With this combination, two-color flow cytometric analysis permits the enumeration and characterization of cells that are actively synthesizing DNA (BrdU incorporation) in terms of their cell cycle position (ie, G0/1, S, or G2/M phase defined by 7-AAD staining intensities).

Reagent	Quantity
Fluorochrome-conjugated anti-BrdU Antibody	One 65-µL vial
BD Cytofix/Cytoperm™ Buffer	One 25-mL bottle
BD Perm/Wash™ Buffer (10X)	Two 25-mL bottles
BD Cytoperm™ Permeabilization Buffer Plus	One 10-mL bottle
7-AAD	One 1-mL vial
BrdU (10 mg/mL)	Five 0.5-mL vials
DNase	Five 300-µL vials

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