Tau 蛋白价格_品牌:StressMarq-丁香通官网

保存条件：-80ºC

英文名：Tau Protein

库存：大量

供应商：StressMarq

规格：100 µg

注射了K18 P301L tau PFF (SPR-330) 的P301L突变型小鼠海马体免疫组化分析，图片显示注射后9周在注射位点出现tau病理性聚集。AT8 (pSer202/pThr205) tau抗体显示缠结内含体. 小图: 阴性对照 . 实验进行于reMYND N.V.

AT8 是抗pSer202/pThr205 tau 蛋白抗体. 注射了非突变小鼠脑干匀浆(BSH) 的Tau P301L 突变小鼠基本没有任何AT8-阳性内含体. 注射了K18 PFF的小鼠脑中 AT8-阳性类缠结内含体的含量要远高于阴性对照. 实验进行于 reMYND N.V.

活性重组 Tau (K18), P301L 突变前体原纤维 (PFFs) 的透射电镜图 (TEM), 放大倍数为 150kx. HV = 80kV.

产品名称	Tau 蛋白
产品描述	Active重组人 Tau (K18), P301L 突变蛋白 PFFs
应用范围	WB, SDS-PAGE, In vivo assay, In vitro assay
浓度	各批次不同，请详见说明书
标记物	无标签
性质	重组
来源物种	人
表达系统	E. coli
氨基酸序列	SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE
蛋白长度	部分蛋白
生物学活性	Active
Full Biological Activity	当活性 Tau PFFs 与活性Tau 单体混合时, 硫黄素 T 荧光曲线显示荧光增强 (相关于 Tau 蛋白纤维聚集).

产品特性

储存缓冲液	10 mM HEPES, 100 mM NaCl pH 7.4
储存温度	-80ºC
运输温度	干冰. 运输: 该产品会与其他同订单产品分开发货.
纯化方式	离子交换层析纯化
特异性	~15.1 kDa
引用该产品	Human Recombinant Tau Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-330)

生物学特性

别名	Active tau PFFs, active tau protein preformed fibrils, active tau aggregates, microtubule-associated protein tau, MAPT, MAP, microtubule-associated protein, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 4, tubulin-associated unit
研究领域	MT 相关蛋白, 神经元标记物, 神经纤维缠结&Tau, 细胞标记物, 轴突标记物, 微管, 神经生物学, 神经衰退疾病, 阿尔茨海默病, 细胞信号传导, 细胞骨架
细胞定位	外周膜蛋白, 微管, 微管相关复合体, 微管蛋白复合体, 树突, 核周区域, 核斑点, 生长锥, 神经元胞体, 神经纤维缠结, 细胞体, 细胞核, 细胞膜, 细胞质, 细胞质侧, 细胞质基质, 细胞质核糖核蛋白体, 细胞骨架, 质膜, 轴突, 轴膜, 轴膜质膜
GeneID	4137
Swiss Prot	P10636
科研背景	Alzheimer’s Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).
参考资料	1. www.alz.org/alzheimers-dementia/facts-figures 2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146–148 (1907) 3. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497. 4. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18. 5. Guo, J. and Lee, M.Y. (2013). FEBS Lett. 587(6): 717-723. 6. Alberici, A. et al. (2004). J Neurol Neurosurg Psychiatry. 75:1607-1610.