产品详情
文献和实验
相关推荐
库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK0422 |
|---|---|
| 产品名称 | Mouse IL-12（P70) ELISA Kit |
| 产品描述 | For quantitative detection of mouse IL-12(P70) in cell culture supernates and serum. |
| 免疫原 | NSO, (M23-S335)+(R23-A215) |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1.5pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | IL12A |
| 基因全名 | interleukin 12A |
| Uniprot ID | F8WI71 |
| 指标别名 | CLMF|CLMF p35|Cytotoxic lymphocyte maturation factor 1|Cytotoxic lymphocyte maturation factor 1 p35|Cytotoxic lymphocyte maturation factor 35 kDa subunit|IL-12 subunit p35|IL-12A|IL12A|IL12A_HUMAN|IL35 subunit|Interleukin 12 alpha chain|Interleukin 12 p35|Interleukin 12 p70|Interleukin-12 subunit alpha|Natural killer cell stimulatory factor 1|Natural killer cell stimulatory factor 1 35 kD subunit|NF cell stimulatory factor chain 1|NFSK|NK cell stimulatory factor chain 1|NKSF1|P35 |
| 背景资料 | Interleukin(IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins(p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits; it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40(protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant mouse IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0422-CAP | 96-well plate precoated with anti- mouse IL-12(p70) antibody | 1 |
| EK0422-ST | Lyophilized recombinant mouse IL-12(p70) standard | 10ng/tubex2 |
| EK0422-DA | Biotinylated anti- mouse IL-12(p70) antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 37 | 150 | 410 | 33 | 147 | 442 |
| Standard deviation | 2.18 | 9.15 | 30.75 | 2.44 | 8.96 | 38.45 |
| CV(%) | 5.9% | 6.1% | 7.5% | 7.4% | 6.1% | 8.7% |
Mouse IL-12（P70) ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.044 | 0.088 | 0.116 | 0.194 | 0.338 | 0.618 | 1.144 | 2.010 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
