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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernatants, serum and plasma (heparin, EDTA).
样本 ：human
规格 ：96T
产品概况
| 货号 | EK0386 |
|---|---|
| 产品名称 | Human IGFBP-3 ELISA Kit |
| 产品描述 | For quantitative detection of human IGFBP3 in cell culture supernatants, serum and plasma (heparin, EDTA). |
| 免疫原 | Expression system for standard: NS0; Immunogen sequence: A27-R291 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <75 pg/ml |
| 检测范围 | 156 pg/ml - 10,000 pg/ml |
| 基因名称 | IGFBP3 |
| 基因全名 | insulin-like growth factor binding protein 3 |
| Uniprot ID | IGFBP3: P17936 |
| 指标别名 | BP 53; IBP 3; IBP3; IGF binding protein 3; IGFBP 3; IGFBP3 |
| 背景资料 | Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression. Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix. IGFBP3 is also located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa. |
| 显色时间 | 15-20分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0386-CAP | 96-well plate precoated with anti- Human IGFBP3 ELISA Kit A antibody | 1 |
| EK0386-ST | Lyophilized recombinant Human IGFBP3 ELISA Kit A standard | 10ng/tube×2 |
| EK0386 -DA | Biotinylated anti- Human IGFBP3 ELISA Kit A antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 271 | 1607 | 5480 | 253 | 1639 | 5548 |
| Standard deviation | 16.53 | 69.1 | 350.72 | 19.73 | 95.06 | 437.8 |
| CV(%) | 6.1% | 4.3% | 6.4% | 7.8% | 5.8% | 7.9% |
Human IGFBP-3 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 15-20分钟)| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.034 | 0.092 | 0.160 | 0.230 | 0.397 | 0.691 | 1.251 | 1.710 |
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