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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum, plasma (heparin, EDTA) and urine.
规格 ：96T
产品概况
| 货号 | EK0384 |
|---|---|
| 产品名称 | Human IGFBP2 ELISA Kit |
| 产品描述 | For quantitative detection of human IGFBP2 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. |
| 免疫原 | NSO, E40-Q328 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 62.5pg/ml-4000pg/ml |
| 基因名称 | IGFBP2 |
| 基因全名 | insulin-like growth factor binding protein 2, 36kDa |
| Uniprot ID | P18065 |
| 指标别名 | BP 2|BP2|IBP 2|IBP-2|IBP2|IBP2_HUMAN|IGF binding protein 2|IGF BP53|IGF-binding protein 2|IGF-BP53|IGFBP-2|IGFBP2|IGFBP53|Insulin Like Growth Factor Binding Protein 2|Insulin like growth factor binding protein 2 36kDa|Insulin like growth factor-binding protein 2 precursor|Insulin-like growth factor-binding protein 2 |
| 背景资料 | Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0384-CAP | 96-well plate precoated with anti- human IGFBP2 antibody | 1 |
| EK0384-ST | Lyophilized recombinant human IGFBP2 standard | 10ng/tubex2 |
| EK0384-DA | Biotinylated anti- human IGFBP2 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 128 | 598 | 1114 | 133 | 553 | 1221 |
| Standard deviation | 5.88 | 47.24 | 61.27 | 7.84 | 54.19 | 83.02 |
| CV(%) | 4.6% | 7.9% | 5.5% | 5.9% | 9.8% | 6.8% |
Human IGFBP2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.137 | 0.218 | 0.283 | 0.422 | 0.691 | 1.201 | 1.916 | 2.423 |
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