产品详情
文献和实验
相关推荐
库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
样本 ：Mouse
规格 ：96T
产品概况
| 货号 | EK0365 |
|---|---|
| 产品名称 | Mouse GM-CSF ELISA Kit |
| 产品描述 | For quantitative detection of mouse GM-CSF in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli, A18-K141 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | CSF2 |
| 基因全名 | colony stimulating factor 2 (granulocyte-macrophage) |
| Uniprot ID | P01587 |
| 指标别名 | Colony stimulating factor|Colony stimulating factor 2 (granulocyte macrophage)|CSF|CSF 2|CSF2|GMCSF|Granulocyte macrophage colony stimulating factor|MGC131935|MGC138897|Molgramostin|Sargramostim |
| 背景资料 | Granulocyte-macrophage colony-stimulating factor(GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene. Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375. The standard product used in this kit is recombinant mouse GM-CSF, consisting of 125 amino acids with the molecular mass of 14.8KDa. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0365-CAP | 96-well plate precoated with anti- mouse GM-CSF antibody | 1 |
| EK0365-ST | Lyophilized recombinant mouse GM-CSF standard | 10ng/tube×2 |
| EK0365-DA | Biotinylated anti- mouse GM-CSF antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 32 | 234 | 480 | 35 | 213 | 448 |
| Standard deviation | 1.34 | 16.38 | 19.68 | 1.54 | 14.91 | 21.05 |
| CV(%) | 4.2% | 7% | 4.1% | 4.4% | 7% | 4.7% |
Mouse GM-CSF ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.007 | 0.041 | 0.098 | 0.172 | 0.465 | 0.760 | 1.502 | 2.104 |
武汉博士德生物工程有限公司
品牌商实名认证
金牌会员
入驻年限：17年
