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库存 ：发货周期：现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma(heparin).
样本 ：Rat
规格 ：96T
产品概况
| 货号 | EK0639 |
|---|---|
| 产品名称 | Rat MMP-2 ELISA Kit |
| 产品描述 | For quantitative detection of rat MMP-2 in cell culture supernates, serum and plasma(heparin). |
| 免疫原 | NSO, A30-C662 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 625pg/ml-40000pg/ml |
| 基因名称 | MMP2 |
| 基因全名 | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
| Uniprot ID | P33436 |
| 指标别名 | 72 kDa gelatinase|72 kDa type IV collagenase|CLG 4|CLG 4A|CLG4|CLG4A|Collagenase type IV A|EC 3.4.24.24|Gelatinase A|Gelatinase alpha|Gelatinase neutrophil|Matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|Matrix metalloproteinase 2|Matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)|Matrix metalloproteinase II|Matrix metalloproteinase-2|MMP 2|MMP II|MMP-2|MMP2|MMP2_HUMAN|MONA|Neutrophil gelatinase|PEX|TBE 1|TBE-1 |
| 背景资料 | Type IV collagenase, 72-kD, is officially designated matrix metalloproteinase-2(MMP2). It is also known as gelatinase, 72-kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases(MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide(cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72KDa. The detected MMP-2 includes zymogen and active enzyme. |
| 显色时间 | 25-30分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0639-CAP | 96-well plate precoated with anti- rat MMP-2 antibody | 1 |
| EK0639-ST | Lyophilized recombinant rat MMP-2 standard | 40ng/tubex2 |
| EK0639-DA | Biotinylated anti- rat MMP-2 antibody | 100ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1093 | 4492 | 23073 | 1098 | 4440 | 21240 |
| Standard deviation | 68.85 | 296.47 | 1107.5 | 90.03 | 368.52 | 1295.64 |
| CV(%) | 6.3% | 6.6% | 4.8% | 8.2% | 8.3% | 6.1% |
Rat MMP-2 ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 25-30分钟)| Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
| O.D. | 0.086 | 0.192 | 0.233 | 0.335 | 0.524 | 0.833 | 1.478 | 2.119 |
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入驻年限：17年
