Ribonuclease Inhibitor核糖核酸酶抑制因子1抗体

Ribonuclease Inhibitor核糖核酸酶抑制因子1抗体

价格: ¥1380 - 2200

品牌:LMAI Bio

货号:LM-7518R

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供应商 :上海联迈生物工程有限公司

库存 :大量

目录编号 :LM-7518R

克隆性 :多克隆

抗原来源 :Rabbit

保质期 :1年

抗体英文名 :Ribonuclease Inhibitor

抗体名 :核糖核酸酶抑制因子1抗体

宿主 :Rabbit

适应物种 :Human, Mouse, Rat, Pig, Cow,

免疫原 :KLH conjugated synthetic peptide derived from hu Ribonuclease Inhibitor:101-200/461

亚型 :IgG

形态 :Lyophilized or Liquid

应用范围 :WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)

浓度 :1mg/ml

保存条件 :Store at -20 °C

规格 :100ul  200ul

Ribonuclease Inhibitor核糖核酸酶抑制因子1抗体
英文名称 Ribonuclease Inhibitor
中文名称 核糖核酸酶抑制因子1抗体
别    名 Placental ribonuclease inhibitor; RNH; RNH1; Placental RNase inhibitor; PRI; RAI; RI antibody Ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1; Ribonuclease/angiogenin inhibitor; RINI_HUMAN; RNase inhibitor; RNH 1; Rnh1 ribonuclease/angiogenin inhibitor 1.  
Specific References  (1)     |     bs-7518R has been referenced in 1 publications.
[IF=1.60] Tang, Y., et al. "Overexpression of ribonuclease inhibitor defines good prognosis and suppresses proliferation and metastasis in human colorectal cancer cells via PI3K/AKT pathway." Clinical and Translational Oncology: 1-8.  IHC-P ;  Human.  
PubMed:25257839
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 细胞生物  信号转导  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from hu Ribonuclease Inhibitor:101-200/461 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis.

Subunit:
Forms high-affinity heterodimers with RNASE1, ANG and RNASE2.

Subcellular Location:
Cytoplasm.

Post-translational modifications:
The N-terminus is blocked.
At least 30 of the 32 cysteine residues are in the reduced form.

Similarity:
Contains 15 LRR (leucine-rich) repeats.

SWISS:
P13489

Gene ID:
6050

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

Blank control(blue): Hela cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). 
Primary Antibody:Rabbit Anti- Ribonuclease Inhibitor antibody(bs-1638R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; 
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); 
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

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