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供应商 :上海联迈生物工程有限公司
库存 :大量
目录编号 :LM-7518R
克隆性 :多克隆
抗原来源 :Rabbit
保质期 :1年
抗体英文名 :Ribonuclease Inhibitor
抗体名 :核糖核酸酶抑制因子1抗体
宿主 :Rabbit
适应物种 :Human, Mouse, Rat, Pig, Cow,
免疫原 :KLH conjugated synthetic peptide derived from hu Ribonuclease Inhibitor:101-200/461
亚型 :IgG
形态 :Lyophilized or Liquid
应用范围 :WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复)
浓度 :1mg/ml
保存条件 :Store at -20 °C
规格 :100ul 200ul
Ribonuclease Inhibitor核糖核酸酶抑制因子1抗体英文名称 | Ribonuclease Inhibitor |
中文名称 | 核糖核酸酶抑制因子1抗体 |
别 名 | Placental ribonuclease inhibitor; RNH; RNH1; Placental RNase inhibitor; PRI; RAI; RI antibody Ribonuclease inhibitor; Ribonuclease/angiogenin inhibitor 1; Ribonuclease/angiogenin inhibitor; RINI_HUMAN; RNase inhibitor; RNH 1; Rnh1 ribonuclease/angiogenin inhibitor 1. |
Specific References (1) | bs-7518R has been referenced in 1 publications. [IF=1.60] Tang, Y., et al. "Overexpression of ribonuclease inhibitor defines good prognosis and suppresses proliferation and metastasis in human colorectal cancer cells via PI3K/AKT pathway." Clinical and Translational Oncology: 1-8. IHC-P ; Human. PubMed:25257839 |
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规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 细胞生物 信号转导 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Pig, Cow, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 50kDa |
细胞定位 | 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from hu Ribonuclease Inhibitor:101-200/461 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and ANG. May play role in redox homeostasis. Subunit: Forms high-affinity heterodimers with RNASE1, ANG and RNASE2. Subcellular Location: Cytoplasm. Post-translational modifications: The N-terminus is blocked. At least 30 of the 32 cysteine residues are in the reduced form. Similarity: Contains 15 LRR (leucine-rich) repeats. SWISS: P13489 Gene ID: 6050 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Ribonuclease Inhibitor) Polyclonal Antibody, Unconjugated (bs-7518R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining. Blank control(blue): Hela cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Ribonuclease Inhibitor antibody(bs-1638R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
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