英文名 ：Twin-Strep-tag® Capture Kit

保质期 ：12

供应商 ：IBA Lifesciences

保存条件 ：2-8 °C, -20 °C

规格 ：1 kit/1 Kit, Maxi

Twin-Strep-tag^® Capture Kit

表面等离子体共振技术（Surface plasmon resonance, SPR）是分析生物分子间结合作用的常用方法，配体蛋白（ligand）需高效的结合在已固定的捕获分子上，从而进行检测。
Twin-Strep-tag^® Capture试剂盒， 适用于使用 Biacore SPR 系统在羧基衍生化传感器芯片上高亲和力（可逆）捕获Twin-Strep-tag^®融合蛋白。
此试剂盒包含Strep-Tactin^®XT（捕获分子）、对照蛋白、固定缓冲液和再生缓冲液。
 

核心优点

• 该配体的间接固定化可防止该配体失活或受固定化过程的影响。此外，不需要对配体进行化学修饰。
• 亲和力高，检测时间可以更长（亲和力pM级别; T1/2= 13 h）
• 晶片可以再生，节约成本。
• 因为Twin-Strep-tag®:Strep-Tactin®XT的高亲和力和特异性，配体能直接在复合物中被捕获，无需样本纯化。

Twin-Strep-tag® Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag® fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-Tactin®XT, SPR Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag® as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-Tactin®XT:Twin-Strep-tag® interaction, which operates with pM affinity. 

The key benefits are:
• Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure
• High affinity enabling measurements over a long period (high affinity in low pM range; T1/2 = 13 h)
• Chip can be regenerated easily by an established procedure – cost effective
• Ligands can be captured directly from complex samples due to the high affinity and specificity of the Twin-Strep-tag®:Strep-Tactin®XT interaction 
The use of unconjugated Strep-Tactin®XT requires a license that is provided with the purchase of the kit as limited use license.
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