SIRT3线粒体乙酰化酶3抗体

供应商 ：上海联迈生物工程有限公司

库存 ：大量

目录编号 ：LM-6105R

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：1年

抗体英文名 ：SIRT3

抗体名 ：线粒体乙酰化酶3抗体

宿主 ：Rabbit

适应物种 ：Human, Mouse, Rat, Dog, Pig, Rabbit,

免疫原 ：KLH conjugated synthetic peptide derived from human SIRT3:201-300/399

亚型 ：IgG

形态 ：Lyophilized or Liquid

应用范围 ：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）

浓度 ：1mg/ml

保存条件 ：Store at -20 °C

规格 ：50ul  100ul  200ul

SIRT3线粒体乙酰化酶3抗体

英文名称	SIRT3
中文名称	线粒体乙酰化酶3抗体
别    名	hSIRT3; hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent deacetylase sirtuin-3, mitochondrial; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIRT 3; SIRT3; SIRT3_HUMAN; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3.
规格价格	50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	50ul  100ul  200ul
研究领域	肿瘤  心血管  细胞生物  细胞周期蛋白  线粒体
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Rabbit,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	43kDa
细胞定位	细胞浆 线粒体
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human SIRT3:201-300/399
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. Function: NAD-dependent protein deacetylase. Activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. Subunit: Interacts with NDUFA9, ACSS1, IDH2 and GDH. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Widely expressed. Post-translational modifications: Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. Similarity: Belongs to the sirtuin family. Contains 1 deacetylase sirtuin-type domain. SWISS: Q9NTG7 Gene ID: 23410 Database links: Entrez Gene: 23410 Human Entrez Gene: 64384 Mouse Omim: 604481 Human SwissProt: Q9NTG7 Human SwissProt: Q8R104 Mouse Unigene: 716456 Human Unigene: 244216 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample:  Liver (Mouse) Lysate at 40 ug Heart (Mouse) Lysate at 40 ug Primary: Anti-SIRT3 (bs-6105R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT3) Polyclonal Antibody, Unconjugated (bs-6105R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.