phospho-CD32B(Tyr292)磷酸化CD32B抗体

phospho-CD32B(Tyr292)磷酸化CD32B抗体

价格: ¥1580

品牌:LMAI Bio

货号:LM-6031R

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供应商 :上海联迈生物工程有限公司

库存 :大量

目录编号 :LM-6031R

克隆性 :多克隆

抗原来源 :Rabbit

保质期 :1年

抗体英文名 :phospho-CD32B(Tyr292)

抗体名 :磷酸化CD32B抗体

宿主 :Rabbit

适应物种 :Human, Mouse, Rat, Dog, Pig, Rabbit, Sheep, Guinea Pig,

免疫原 :KLH conjugated Synthesised phosphopeptide derived from human CD32B around the phosphorylation site of Tyr291.:231-310/310

亚型 :IgG

形态 :Lyophilized or Liquid

应用范围 :WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)

浓度 :1mg/ml

保存条件 :Store at -20 °C

规格 :100ul  

phospho-CD32B(Tyr292)磷酸化CD32B抗体
英文名称 phospho-CD32B(Tyr292)
中文名称 磷酸化CD32B抗体
别    名 CD32B(phospho Y292); CD32B; FCGR2B; IGFR2; Lym 1; CD32; CD32 antigen; CDw32; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for; Fc gamma receptor IIB; Fc gamma RII; Fc gamma RIIB; Fc-gamma RII; Fc-gamma RII-b; Fc-gamma-RIIb; FCG2; FCG2B_HUMAN; Fcgr2; FCRII; FcRII-b; IgG Fc receptor II beta; IgG Fc receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II; Low affinity immunoglobulin gamma Fc region receptor II-b; Ly-17; Lymphocyte antigen 17.  
规格价格 100ul/1580元 购买        大包装/询价
说 明 书 100ul  
产品类型 磷酸化抗体 
研究领域 细胞生物  免疫学  细胞表面分子  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Rabbit, Sheep, Guinea Pig, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 31kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human CD32B around the phosphorylation site of Tyr291.:231-310/310 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010].

Function:
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.

Subunit:
Isoform IIB1 interacts with measles virus N protein. N protein is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.

Post-translational modifications:
Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.

Similarity:
Contains 2 Ig-like C2-type (immunoglobulin-like).

SWISS:
P31994

Gene ID:
2213

Database links:

Entrez Gene: 2213 Human

Omim: 604590 Human

SwissProt: P31994 Human

Unigene: 654395 Human

Unigene: 713617 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-CD32B(Tyr292) (bs-6031R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 36 kD


Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CD32B(Tyr292)) Polyclonal Antibody, Unconjugated (bs-6031R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

phosphopeptide non phosphopeptide

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