产品详情
文献和实验
相关推荐
供应商 :上海联迈生物工程有限公司
库存 :大量
目录编号 :LM-5174R
克隆性 :多克隆
抗原来源 :Rabbit
保质期 :1年
抗体英文名 :phospho-APC1 (Ser377)
抗体名 :磷酸化细胞周期末期促进复合蛋白APC1抗体
宿主 :Rabbit
适应物种 :Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
免疫原 :KLH conjugated Synthesised phosphopeptide derived from human ANAPC1 around the phosphorylation site of Ser377:NQ(p-S)PK
亚型 :IgG
形态 :Lyophilized or Liquid
应用范围 :ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
浓度 :1mg/ml
保存条件 :Store at -20 °C
规格 :100ul
phospho-APC1 (Ser377)磷酸化细胞周期末期促进复合蛋白APC1抗体英文名称 | phospho-APC1 (Ser377) |
中文名称 | 磷酸化细胞周期末期促进复合蛋白APC1抗体 |
别 名 | Apc1 (phospho S377); p-Apc1 (phospho S377); phospho-ANAPC1(Ser377); ANAPC 1; ANAPC1; Anaphase promoting complex subunit 1; anaphase-promoting complex 1 (meiotic checkpoint regulator); Anaphase-promoting complex subunit 1; Apc 1; APC1; APC1_HUMAN; Cyclosome subunit 1; MCPR; Meiotic checkpoint regulator; Mitotic checkpoint regulator; Protein Tsg 24; Protein Tsg24; Testis-specific gene 24 protein; TSG 24; TSG24. |
规格价格 | 100ul/1580元 购买 大包装/询价 |
说 明 书 | 100ul |
产品类型 | 磷酸化抗体 |
研究领域 | 细胞生物 免疫学 细胞周期蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 212kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human ANAPC1 around the phosphorylation site of Ser377:NQ(p-S)PK |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation. [provided by RefSeq, Dec 2011]. Function: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Subunit: The APC/C is composed of at least 12 subunits. Post-translational modifications: Phosphorylated. Phosphorylation on Ser-355 occurs specifically during mitosis. Similarity: Belongs to the APC1 family. Contains 4 PC repeats. SWISS: Q9H1A4 Gene ID: 64682 Database links: Entrez Gene: 64682 Human Entrez Gene: 535565 Cow Entrez Gene: 475750 Dog Entrez Gene: 17222 Mouse Omim: 608473 Human SwissProt: E1BAB6 Cow SwissProt: E2RLM2 Dog SwissProt: Q9H1A4 Human
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 | Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-APC1 (Ser377)) Polyclonal Antibody, Unconjugated (bs-5174R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. |
上海联迈生物工程有限公司
品牌商实名认证
钻石会员
入驻年限:8年