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库存 :100
英文名 :Virus DNA/RNA Extraction Kit II
CAS号 :VR050/VR100/VR300
保质期 :2年
供应商 :北京智杰方远
保存条件 :RT
规格 :100T
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英文名 | 中文名 | 品牌 | 货号 | 规格 |
Virus DNA/RNA Extraction Kit II | 病毒核酸纯化试剂盒 | Geneaid Biotech | VR100 | 100T |
Virus DNA/RNA Extraction Kit II VR050/VR100/VR300
The Viral Nucleic Acid Extraction Kit II was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. The efficient glass fiber spin column system is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 101-109 copies of viral DNA/RNA can be purified from up to 200 µl samples within 20 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.
Advantages (Cat. # VR050, VR100, VR300)
- High Sensitivity: virus RNA/DNA can be successfully extracted and detected from as low as 10E1 copy number!
- Purify virus DNA or virus RNA in 20 minutes!
- Sample Volume: up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures
- Spin Columns: glass fiber membrane optimized for virus DNA and virus RNA purification
- Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free
- Elution Volume: 50 µl
- Storage: dry at room temperature (15-25ºC)
RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS)
Components
- VB Lysis Buffer
- AD Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- VB Columns
- 2 ml Collection Tubes
The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.
Viral Nucleic Acid Extraction Kit II Functional Test Data
Figure 2. Hepatitis A Virus (HAV) RNA was extracted using the Geneaid Viral Nucleic Acid Extraction Kit II. The purified RNA was analyzed by electrophoresis on a 1% agarose gel. M: Geneaid 1 Kb DNA Ladder 1: HAV from original serum 2: HAV from 10X diluted serum 3: HAV from 100X diluted serum
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Figure 3. HBV (DNA), HCV (RNA), HIV (RNA), and HTLV (RNA) were purified from 200 µl of positive clinical serum samples using the Viral Nucleic Acid Extraction Kit II. Real-time qPCR and 1-step qRT-PCR reactions were then conducted using the ABI 7300 Sequence Detection System (3 replications of each copy number). Serum samples containing various amounts of DNA/RNA viruses ranging from 10E1 to 10E6 copies/ml were successfully detected and identified. The low Ct values indicate a high number of target nucleic acid in the sample. |
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Related Virus DNA/RNA Purification Products
Product Name | RXNS | Catalogue Number |
Viral Nucleic Acid Extraction Kit II (up to 200 µl sample) | 50, 100, 300 | VR050/100/300 |
Viral Nucleic Acid Extraction Kit III (up to 1 ml sample) | 50, 100, 300 | VI050/100/300 |
Plant Virus RNA Kit | 50, 100 | PVR050/100 |
仅供科研使用
产品编号
VR050, VR100, VR300
完整说明书(英文)下载
如果您是第一次使用本产品或对本产品的操作步骤不熟悉,请您经由扫描QR code下载并详阅完整说明书
1. 处理材料
取200 μl样本(例如血浆,血清,淋巴液或经病毒感染的细胞培养液)转移到1.5 ml微量离心管中.
注意:样本不足200 μl可加缓冲液PBS补足.
加入400 μl缓冲液VB Lysis,使用涡旋振荡器振荡混匀,室温放置10分钟.
2. 吸附
加入450 μl缓冲液AD(使用前请先检查是否已加入无水乙醇),充分摇动混匀. 将吸附柱VB放入2 ml收
集管中. 将600 μl所得溶液加入吸附柱VB中,以14-16,000×g离心1分钟,倒掉收集管中的废液,将吸附柱
放回收集管中. 将剩余的溶液加入吸附柱VB中,以14-16,000×g离心1分钟,丢弃收集管,将吸附柱放入
新的收集管中.
3. 漂洗
向吸附柱VB中加入400 μl漂洗液W1,以14-16,000 ×g离心30秒,倒掉收集管中的废液,将吸附柱放入收集
管中. 向吸附柱VB中加入600 μl漂洗液Wash(使用前请先检查是否已加入无水乙醇),以14-16,000×g离
心30秒,倒掉收集管中的废液,将吸附柱VB放入收集管中. 以14-16,000 ×g离心3分钟将吸附柱中残余的
漂洗液去除.
注意:漂洗液中乙醇的残留会影响后续实验.
4. 洗脱
将吸附柱VB放入一个RNase-free的离心管中,向吸附膜中间位置悬空滴加50 μl洗脱缓冲液RNase-free
water,室温放置3分钟后,以14-16,000 ×g离心1分钟收集病毒DNA/RNA溶液。
北京智杰方远科技有限公司成立于2011年是一家细胞培养产品专业供应商,专注细胞生物学试剂开发、销售、服务的高新技术企业。主要代理产品如下: 1.进口胎牛血清:美国GIBCO、德国PAN-biotech、以色列BI、澳大利亚Ausgene-X、美国GEMINI、美国Hyclone等。 2.TAKARA、Biolog微生物鉴定板、Borer清洗产品、台湾旭基。 3.MP Bio、日本同仁、Thermo、SIGMA、QIAGEN、MERCK、LEICA、Millipore、Whatman等。好产品+好价格+好服务为经营理念,希望不断发展的智杰方远成为您最佳的选择!
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