PI 3 Kinase Class 3磷脂酰肌醇激酶3催化亚单位3抗体

供应商 ：上海联迈生物工程有限公司

库存 ：大量

目录编号 ：LM-4159R

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：1年

抗体英文名 ：PI 3 Kinase Class 3

抗体名 ：磷脂酰肌醇激酶3催化亚单位3抗体

宿主 ：Rabbit

适应物种 ：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,

免疫原 ：KLH conjugated synthetic peptide derived from human PIK3C3/PI3 kinase type 3:301-400/887

亚型 ：IgG

形态 ：Lyophilized or Liquid

应用范围 ：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）

浓度 ：1mg/ml

保存条件 ：Store at -20 °C

规格 ：50ul  100ul  200ul

PI 3 Kinase Class 3磷脂酰肌醇激酶3催化亚单位3抗体

英文名称	PI 3 Kinase Class 3
中文名称	磷脂酰肌醇激酶3催化亚单位3抗体
别    名	PI 3 Kinase Class 3; Phosphatidylinositol 3 kinase catalytic subunit type 3; Phosphatidylinositol 3 kinase class 3; Phosphatidylinositol 3 kinase p100 subunit; Phosphoinositide 3 kinase class 3; PI3 kinase type 3; PI3K type 3; PIK3C3; PtdIns 3 kinase type 3; Vps 34; Vps34; hVps34; MGC61518; PIK3C3; PI3 Kinase p100; PK3C3_HUMAN.
规格价格	50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	50ul  100ul  200ul
研究领域	免疫学  信号转导  激酶和磷酸酶
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	98kDa
细胞定位	细胞浆
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthetic peptide derived from human PIK3C3/PI3 kinase type 3:301-400/887
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: PI 3 Kinase Class 3 is a member of the PI3/PI4-kinase family. It is the catalytic subunit of the PI3K complex. It is involved in the transport of lysosomal enzyme precursors to lysosomes. It is ubiquitously expressed, with a highest expression in skeletal muscle. Function: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes. Subunit: Heterodimer. This subunit, part of a complex composed of regulatory and catalytic subunits, associates with regulatory subunit PIK3R4. Forms a complex with BECN1, PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. In this complex, presence of UVRAG and ATG14 are mutually exclusive. Part of a complex composed of PIK3R4 and PIK3CB (By similarity). Interacts with RAB7A in the presence of PIK3R4. Subcellular Location: Midbody. Late endosome. Tissue Specificity: Ubiquitously expressed, with a highest expression in skeletal muscle. Similarity: Belongs to the PI3/PI4-kinase family.  Contains 1 C2 PI3K-type domain.  Contains 1 PI3K/PI4K domain.  Contains 1 PIK helical domain.  SWISS: Q8NEB9 Gene ID: 5289 Database links: Entrez Gene: 5289 Human Entrez Gene: 225326 Mouse Entrez Gene: 65052 Rat Omim: 602609 Human SwissProt: Q8NEB9 Human SwissProt: Q6PF93 Mouse SwissProt: O88763 Rat Unigene: 464971 Human Unigene: 194127 Mouse Unigene: 30010 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: brain(mouse) Lysate at 40 ug Primary: Anti-PI 3 Kinase Class 3 (bs-4159R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98kD Observed band size: 98 kD Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PI 3 Kinase Class 3) Polyclonal Antibody, Unconjugated (bs-4159R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-PI 3 Kinase Class 3 Polyclonal Antibody, Unconjugated(bs-4159R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (Black line): HUVEC (Black).  Primary Antibody (green line): Rabbit Anti-PI 3 Kinase Class 3 antibody (bs-4159R)  Dilution: 1μg /10^6 cells;  Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test.  Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.