Calbindin D-9k-60µg

保存条件 ：Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.

供应商 ：安诺伦（北京）生物科技有限公司

规格 ：60 µg

研究领域：Calbindin

靶点/蛋白质：Rat recombinant calbindin D-9k (recombinant) produced in Escherichia Coli.

来源：Recombinant rat

官网链接：https://www.swant.com/pdfs/Calbindin_9k_recombinant-rat.pdf

商品关键词：Calbindin D-9k,rCB9,Swant

Purification: Ni-chelate chrom.

Lot No: 5

Reconstitution: 10 ug protein reconstituted with 25 ul PBS (0.4 ug/ul) 60 ug protein reconstituted with 150 ul PBS

Purification: E. coli containing a His6-fusion protein were disrupted in a buffer containing 6M GuHCl/0.1 M NaH2PO4. After a low speed centrifugation to remove cell debris, the supernatant was applied to the charged NTA resin column, equilibrated in disruption buffer, before the column being washed with the same buffer. Afterwards, the column was washed and the HIS6- fusion proteins eluted by decreasing the pH of the eluent (8 M Urea /0.1 M Na2HPO4) in a stepwise manner (pH 6.3 / 5.9 / 4 / 2). The protein was dialysed against phosphate buffered saline, 0.1 M, pH 7.3 (PBS).


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