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保存条件 :Reconstitute with 10 ul bidistilled water. Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.
供应商 :安诺伦(北京)生物科技有限公司
规格 :10 µg
研究领域:Others靶点/蛋白质:S100A6 (calcyclin) antigen (10 ug)
来源:Mouse
存储溶液:lyophilized in ddH2O
官网链接:https://www.swant.com/pdfs/S100A6_calcyclin.pdf
商品关键词:S100A6 Calcyclin,pCAcy,Swant
简单描述:This protein has been purified from mouse Ehrlich ascites cells by HPLC (see Kuznicki et al. (1989) Biochem. J. 263: 951-956). This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For fluid-phase adsorption in immunohistochemistry we suggest the following procedure:- Dilute 1 µl of the antiserum against S100A6 (calcyclin) (Swant) in 5 ml of the usual buffer forimmunohistochemistry (final dilution 1:5'000).- Add 1 µg of the purified protein to 1 ml of the diluted antibody solution and mix well.- Incubate for at least 6 hours in the cold.- Apply to tissue-sections and incubate for 3 days.- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.
Purification: Chromatogr.
Lot No: 2.’96
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入驻年限:9年