Parvalbumin

保存条件 ：Reconstitute with 10 or 100 ul bidistilled water. Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.

供应商 ：安诺伦（北京）生物科技有限公司

规格 ：10 µg

研究领域：Parvalbumin

靶点/蛋白质：Rat parvalbumin (recombinant) produced in Escherichia Coli.

来源：Recombinant rat

存储溶液：lyophilized (10 or 100 ug in 100 ul of: 10 mM Tris-base, 70 mM NaCl, 1 mM EDTA, 1mM 脽-mercaptoethanol, pH 7.4).

官网链接：https://www.swant.com/pdfs/Parvalbumin_recombinant-rat.pdf

商品关键词：Parvalbumin,rPV10,Swant

简单描述：This recombinant protein has been purified by FPLC.This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: - Dilute 1 ul of the mon oclonal antibody 235 (Swant) against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 ug of the recombinant protein to 1 ml of the diluted antibody solution and mix well.- Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

Purification: HPLC

Lot No: 3.'94


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