Human VEGF ELISA Kit

价格： ￥2280

品牌：BOSTER 品牌认证

货号：EK0539

产品详情

产品概况

货号	EK0539
产品名称	Human VEGF ELISA Kit
产品描述	For quantitative detection of human VEGF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
免疫原	NSO,A27-R191
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<1pg/ml
检测范围	31.2pg/ml-2000pg/ml
基因名称	VEGFA
基因全名	vascular endothelial growth factor A
Uniprot ID	P15692
指标别名	MGC70609\|MVCD1\|Vascular endothelial growth factor\|Vascular endothelial growth factor A\|Vascular permeability factor\|VEGF A\|VEGFA\|VPF
背景资料	Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis.VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1. The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12.
显色时间	15-20分钟

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0539-CAP	96-well plate precoated with anti- human VEGF antibody	1
EK0539-ST	Lyophilized recombinant human VEGF standard	10ng/tubex2
EK0539-DA	Biotinylated anti- human VEGF antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

1.96孔读板仪器。

2.自动洗板机.

3.移液器和枪头。建议使用多通道移液器/排枪。

4.Eppendorf 管。

5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	72	270	946	76	254	955
Standard deviation	4.53	21.33	59.59	5.92	24.38	74.49
CV(%)	6.3%	7.9%	6.3%	7.8%	9.6%	7.8%