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库存 ：4周
供应商 ：上海研卉生物科技有限公司
规格 ：96T
人内皮素Endothelin-1 ELISA试剂盒RayBio® Human Endothelin-1 ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
Human Endothelin-1 ELISA
Antigen Information
Accession Number:
- P05305
Gene ID:
- 1906
Gene Symbols:
- EDN1
Protein Name & Synonyms:
Endothelin-1 (Preproendothelin-1) (PPET1) [Cleaved into: Endothelin-1 (ET-1) Big endothelin-1]
Target Species:
- Human
Assay Format
Detection Range:
10 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
10 pg/ml
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Specificity:
The antibody pair provided in this kit recognizes Human Endothelin-1 (EDN1)
Number of Targets Detected:
1
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Plasma
- Serum
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Pahari, Dipak R., et al. "Effect of Minimized Perfusion Circuit on Brain Injury Markers Carnosinase and Brain?Type Fatty Binding Protein in Coronary Artery Bypass Grafting Patients."?Artificial organs?37.2 (2013): 128-135.
Species: Human
Sample type: Serum - Said, Neveen, et al. "Tumor endothelin-1 enhances metastatic colonization of the lung in mouse xenograft models of bladder cancer."?The Journal of clinical investigation?121.1 (2011): 132-147.
Species: Human
Sample type: Conditioned Media
- Pahari, Dipak R., et al. "Effect of Minimized Perfusion Circuit on Brain Injury Markers Carnosinase and Brain?Type Fatty Binding Protein in Coronary Artery Bypass Grafting Patients."?Artificial organs?37.2 (2013): 128-135.
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