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库存 ：发货周期:现货
供应商 ：博士德生物
应用 ：cell culture supernates, serum and plasma(heparin, EDTA, citrate).
规格 ：96T
产品概况
| 货号 | EK0525 |
|---|---|
| 产品名称 | Human TNFα ELISA Kit |
| 产品描述 | For quantitative detection of human TNFα in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
| 免疫原 | E.coli,V77-L233 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | TNF |
| 基因全名 | tumor necrosis factor |
| Uniprot ID | TNF: P01375 |
| 指标别名 | APC1 protein|Cachectin|DIF|soluble form|Tnf|TNF a|TNF alpha|TNF macrophage derived|TNF monocyte derived|TNF superfamily member 2|TNF-a|TNF-alpha|TNFA_HUMAN|TNFSF2|Tumor necrosis factor|Tumor necrosis factor alpha|Tumor necrosis factor ligand superfamily member 2 |
| 背景资料 | Tumor necrosis factor-alpha(TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer. Human Tumor Necrosis Factor(TNF) and Lymphotoxin(TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology. TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro. TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids. TNF-alpha has been mapped to human chromosome 6. |
| 显色时间 | 20-25分钟 |
试剂盒内容
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0525-CAP | 96-well plate precoated with anti- human TNF alpha antibody | 1 |
| EK0525-ST | Lyophilized recombinant human TNF alpha standard | 10ng/tube×2 |
| EK0525 -DA | Biotinylated anti- human TNF alpha antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 82 | 201 | 14 | 75 | 186 |
| Standard deviation | 0.76 | 5.49 | 12.66 | 0.75 | 5.55 | 11.9 |
| CV (%) | 4.8 | 6.7 | 6.3 | 5.4 | 7.4 | 6.4 |
Human TNFα ELISA Kit的典型数据示例（取自最近的一批次质检数据）
(TMB 孵育条件： 37°C， 20-25分钟)| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.091 | 0.158 | 0.197 | 0.320 | 0.548 | 0.897 | 1.576 | 2.047 |
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入驻年限：17年
