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标记物 ：Human IL-12 p70 ELISA
检测方法 ：elisa
供应商 ：上海研卉生物科技有限公司
库存 ：4周
适应物种 ：人
检测限 ：1 pg/ml - 600 pg/ml
样本 ： cell culture supernatants, plasma, and serum samples.
应用 ：科研
规格 ：96T
IL-12 ELISA (p70)
人白介素12(IL-12/P70)ELISA试剂盒ELH-IL12P70-1
RayBio® Human IL-12 p70 ELISA Kit for cell culture supernatants, plasma, and serum samples.
人白介素12文献
- Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
Species: Human
Sample type: Plasma - Abdallah M., et al. Serum Th1/Th2 and Macrophage Lineage Cytokines in Leprosy; Correlation with Circulating CD4+ CD25highFoxP3+ T-regs Cells. Experimental Dermatology, August 2014 Accepted Article, doi:10.1111/exd.12529
Species: Human
Sample type: Serum - Santarelli E., Gonnella R., Giovenale G., Cuomo L., Capobianchi A., et al. STAT3 activation by KSHV correlates with IL-10, IL-6 and IL-23 release and an autophagic block in dendritic cells. Scientific Reports, 2014.4 article 4241, doi:10.1038/srep04241
Species: Human
Sample type: Conditioned Media - Hashad RA, Hamed NA, El Gharabawy MM, et al. Interleukins 12 and 13 levels among beta-thalassaemia major patients. East Mediterr Health J. 2013;19(2):181-185.
Species: Human
Sample type: Conditioned Media - Salama, Ragaa HM, et al. "Psoriasin: a novel marker linked obesity with psoriasis."?Disease markers?34.1 (2013): 33-39.
Species: Human
Sample type: Serum - Suga, Takayuki, et al. "Aggregatibacter actinomycetemcomitans lipopolysaccharide stimulated epithelial cells produce interleukin-15 that regulates T cell activation."?Archives of oral biology?58.10 (2013): 1541-1548.
Species: Human
Sample type: Conditioned Media - Mahdi, Nadham Kadham, Abbas Fadhel Ghdbhan, and Murtadh Mohammad Saleh. "Pro-inflammatory Cytokines for Evaluation of the Diagnostic Performance for the Urinary Bladder Cancer."?Austral-Asian Journal of Cancer?12.3 (2013): 169-173.
Species: Human
Sample type: Serum - Bose, Purabi Deka, et al. "High viral load and deregulation of the progesterone receptor signaling pathway: association with hepatitis E-related poor pregnancy outcome."?Journal of hepatology?54.6 (2011): 1107-1113.
Species: Human
Sample type: Serum - RA, Harfoush, and Elgharabawy MM. "Levels of interleukins 12 (IL-12) and 13 (IL-13), hepatitis B and C serology, and blood cultures among acute myeloid leukemia (AML) patients in Egypt."?Journal of Venomous Animals and Toxins including Tropical Diseases?17.3 (2011): 293-299.
Species: Human
Sample type: Conditioned Media - Sabatino A, Pickard K, Rampton D, Kruidenier L, Rovedatti L ,et all.Blockade of transforming growth factor b upregulates T-box transcription factor T-bet, and increases T helper cell type 1 cytokine and matrix metalloproteinase-3 production in the human gut mucosa. Gut.2008;57:605-612 doi:10.1136/gut.2007.130922
Species: Human
Sample type:
Antigen Information
Accession Number:
- P29459
Gene ID:
- 3592
Gene Symbols:
- IL12A
- NKSF1
Protein Name & Synonyms:
Interleukin-12 subunit alpha (IL-12A) (Cytotoxic lymphocyte maturation factor 35 kDa subunit) (CLMF p35) (IL-12 subunit p35) (NK cell stimulatory factor chain 1) (NKSF1)
Target Species:
- Human
Assay Format
Detection Range:
1 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1 pg/ml
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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Number of Targets Detected:
1
Species Detected:
- Human
Compatible Sample Types:
- Cell Culture Supernatants
- Plasma
- Serum
Design Principle:
- Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
- Quantitative
Solid Support:
96-well Microplate
Product Specifications
Size:
1, 2, or 5 x 96-Well Strip Microplate Kit
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
上海研卉生物科技有限公司
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入驻年限：10年
