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库存 :发货周期:现货
供应商 :博士德生物
应用 :cell culture supernates, serum, plasma(EDTA) and urine.
样本 :Mouse
规格 :96T
产品概况
货号 | EK0515 |
---|---|
产品名称 | Mouse TGF beta 1 ELISA Kit |
产品描述 | For quantitative detection of activated mouse TGF beta1 in cell culture supernates, serum, plasma(EDTA) and urine. |
免疫原 | CHO, A279-S390 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <2pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | TGFB1 |
基因全名 | transforming growth factor, beta 1 |
Uniprot ID | P04202 |
指标别名 | Camurati-Engelmann disease|CED|DPD1|LAP|Latency-associated peptide|TGF beta 1 protein|TGF-beta-1|TGFB|tgfb1|TGFB1_HUMAN|TGFbeta|Transforming growth factor beta 1 |
背景资料 | Transforming growth factor-beta1(TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide(beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa. |
显色时间 | 25-30分钟 |
试剂盒内容
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0515-CAP | 96-well plate precoated with anti- mouse TGF beta 1 antibody | 1 |
EK0515-ST | Lyophilized recombinant mouse TGF beta 1 standard | 10ng/tubex2 |
EK0515-DA | Biotinylated anti- mouse TGF beta 1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
需要用户自己提供的器材和试剂
- 1.96孔读板仪器。
- 2.自动洗板机.
- 3.移液器和枪头。建议使用多通道移液器/排枪。
- 4.Eppendorf 管。
- 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
批内差/批间差
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 36 | 169 | 499 | 33 | 183 | 484 |
Standard deviation | 1.47 | 11.66 | 31.43 | 1.55 | 12.99 | 34.36 |
CV(%) | 4.1% | 6.9% | 6.3% | 4.7% | 7.1% | 7.1% |
Mouse TGF beta 1 ELISA Kit的典型数据示例(取自最近的一批次质检数据)
(TMB 孵育条件: 37°C, 25-30分钟)Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.041 | 0.131 | 0.214 | 0.365 | 0.627 | 1.068 | 1.624 | 2.298 |
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入驻年限:17年