phospho-SMAD5 (Ser463 + Ser465)磷酸化细胞信号转导分子SMAD5抗体

供应商 ：上海联迈生物工程有限公司

库存 ：大量

目录编号 ：LM-19918R

克隆性 ：多克隆

抗原来源 ：Rabbit

保质期 ：1年

抗体英文名 ：phospho-SMAD5 (Ser463 + Ser465)

抗体名 ：磷酸化细胞信号转导分子SMAD5抗体

宿主 ：Rabbit

适应物种 ：Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig,

免疫原 ：KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465:IS(p-S)V(p-S)

亚型 ：IgG

形态 ：Lyophilized or Liquid

应用范围 ：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

浓度 ：1mg/ml

保存条件 ：Store at -20 °C

规格 ：100ul  200ul

phospho-SMAD5 (Ser463 + Ser465)磷酸化细胞信号转导分子SMAD5抗体

英文名称	phospho-SMAD5 (Ser463 + Ser465)
中文名称	磷酸化细胞信号转导分子SMAD5抗体
别    名	SMAD5 (phospho S463 + S465); p-SMAD5 (phospho S463 + S465); DKFZp781C1895; DKFZp781O1323; Dwfc; hSmad5; JV5 1; JV5-1; MAD homolog 5; MAD, mothers against decapentaplegic homolog 5; MADH 5; MADH5; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against DPP homolog 5; MusMLP; SMA and MAD related protein 5; SMAD 5; SMAD family member 5; SMAD, mothers against DPP homolog 5; Smad5; Smad5; SMAD5_HUMAN.
规格价格	100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书	100ul  200ul
研究领域	细胞生物  发育生物学  信号转导  干细胞  生长因子和激素  转录调节因子  表观遗传学
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分 子 量	52kDa
细胞定位	细胞核 细胞浆
性    状	Lyophilized or Liquid
浓    度	1mg/ml
免 疫 原	KLH conjugated synthesised phosphopeptide derived from human SMAD5 around the phosphorylation site of Ser463 + Ser465:IS(p-S)V(p-S)
亚    型	IgG
纯化方法	affinity purified by Protein A
储 存 液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). Subcellular Location: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Tissue Specificity: Ubiquitous. Post-translational modifications: Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.  Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. Similarity: Belongs to the dwarfin/SMAD family.  Contains 1 MH1 (MAD homology 1) domain.  Contains 1 MH2 (MAD homology 2) domain. SWISS: Q99717 Gene ID: 4090 Database links: Entrez Gene: 4090 Human Entrez Gene: 17129 Mouse Entrez Gene: 59328 Rat Omim: 603110 Human SwissProt: Q99717 Human SwissProt: P97454 Mouse SwissProt: Q9R1V3 Rat Unigene: 167700 Human Unigene: 272920 Mouse Unigene: 146857 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-p-SMAD5(Ser463+Ser465)(bs-19918R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52 kD Observed band size: 52 kD Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-SMAD5) Polyclonal Antibody, Unconjugated (bs-19918R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.