库存 ：1

供应商 ：北京博蕾德生物科技有限公司

规格 ：96T

              Human Procollagen I N-Terminal Propeptide (PINP) ELISA kit

Format

96-Well Plate

Species Reactivity

Homo sapiens (Human)

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Detection Range

78-5000 pg/mL

Sensitivity

33 pg/mL

Estimated Turnaround Time

7-11 business days

Target Name

Procollagen I N-Terminal Propeptide

Target Synonyms

P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen I N-Terminal Propeptide were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV<10%;Inter-Assay: CV<12%

Detection Method

Colorimetric

Assay Time

3 hours

Application

ELISA

Assay Type

Double-antibody Sandwich

Shipping Condition

Ice packs

Storage

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Expiry Date

8 months

UniProt ID

P02452

Specificity

This assay has high sensitivity and excellent specificity for detection of Procollagen I N-Terminal Propeptide

Cross Activity

No significant cross-reactivity or interference between Procollagen I N-Terminal Propeptide and analogues was observed.

Assay Procedure

1.Prepare all reagents, samples and standards; 2.Add 100 µL standard or sample to each well. Incubate 2 hours at 37°C; 3.Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4.Aspirate and wash 3 times; 5.Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6.Aspirate and wash 5 times; 7.Add 90 µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8.Add 50 µL Stop Solution. Read at 450 nm immediately.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Restrictions

For Research Use Only. Not for use in vitro diagnostic procedures, drug use, or for administration to humans or animals.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Quality Assurance

Biomatik ELISA kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in The kitbefore experiment.

Research Area

Metabolic pathway, Hepatology, Bone metabolism